{"product_id":"immortalized-human-osteoarthritis-cartilage-mesenchymal-stem-cells-oasc5-bhc10900607","title":"Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThese Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) are a robust model for investigating osteoarthritis (OA) pathogenesis and developing therapeutric interventions for OA. Derived from human articular cartilage of osteoarthritis patients, these cells are immortalized to enhance longevity and stability.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) is supplied as an immortalized cell line derived from Human cartilage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS + 100mM HEPES (TM058) + 2mM L-Glutamine (G275) + 0.1 mM Ascorbic acid + 0.1mM Sodium Pyruvate (TM057) + 2.7μM L-glucose + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThus enabling extensive experimental manipulation without the typical constraints of primary cell cultures. OASC5 cells display a unique stellate morphology, intermediate between chondrocytes and bone-marrow derived mesenchymal stem cells. The cells express MSC markers such as CD166, CD105, and CD90 under specific conditions, affirming their identity as mesenchymal stem cells and are capable of differentiating into chondrocytes, osteoblasts, and adipocytes, offering a versatile tool for studying various aspects of cell differentiation. Donor\/background information provided for this product: Adult, Pooled male (61) and female (69), Osteoarthritic articular cartilage.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS + 100mM HEPES (TM058) + 2mM L-Glutamine (G275) + 0.1 mM Ascorbic acid + 0.1mM Sodium Pyruvate (TM057) + 2.7μM L-glucose + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:3\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180504310125,"sku":"T0752","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/54bPBRW3bjd682zkEfrm8RDKeZbe0XsXYxvngT5Q.png?v=1774957765","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-osteoarthritis-cartilage-mesenchymal-stem-cells-oasc5-bhc10900607","provider":"BioHippo","version":"1.0","type":"link"}