{"product_id":"immortalized-human-pancreatic-mesenchymal-stem-cells-negative-ras-bhc10900646","title":"Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Human Pancreatic Mesenchymal Stem Cells - Ras is a immortalized cell line supplied in frozen format and associated with Human pancreas biology.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras is supplied as an immortalized cell line derived from Human pancreas.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in cell fate, differentiation, and expansion. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eQC:\u003c\/strong\u003e Real Time PCR was used to quantify Ras gene expression in immortalized cell line.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e \u003cp\u003e1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/p\u003e\n\u003cp\u003e2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003c\/p\u003e\n\u003cp\u003e3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003c\/p\u003e\n\u003cp\u003e4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003c\/p\u003e\n\u003cp\u003e5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.\u003c\/p\u003e\n\u003cp\u003e6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period.\"\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180504801645,"sku":"T0170","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/UAiMf0PZV3Mi7rElTOgimdhfIIN4cPlzAHK51d91.png?v=1774957765","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-pancreatic-mesenchymal-stem-cells-negative-ras-bhc10900646","provider":"BioHippo","version":"1.0","type":"link"}