{"product_id":"immortalized-human-peripheral-blood-cd4-positive-helper-t-cell-line-negative-sv40-bhc10902170","title":"Immortalized Human Peripheral Blood CD4+ Helper T Cell Line - SV40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line is derived from human peripheral blood CD4+ helper T cells, which are crucial for orchestrating immune responses. Cells were immortalized via the introduction of the SV40 (Simian Virus 40) large T antigen.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Peripheral Blood CD4+ Helper T Cell Line - SV40 is supplied as an immortalized cell line derived from Human blood (immune).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, Round\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal initiation of healthy cell cultures, we recommend using a 24-well plate. Once established, cells can be expanded in PriCoat™ T25 Flasks (G299) for continued growth. PrimGrow T Xpan™ Medium Kit (TM182) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. T Xpan™ Cell Activator (CD3\/CD28\/CD2) (TM139) is required for T cell activation upon thawing. Note 1: refer to the detailed \"Thawing Protocol\" and \"Subculturing Protocol\" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. Note 2: The post-thaw viability of cells can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in 24-well plate and activate before transferring to other culture vessels. Always supplement with fresh reagents. Note 3: We recommend cryopreserving early-passage activated cells as a backup stock for your experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Serial passaging and retroviral transduction with retroviruses carrying the SV40 gene.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis modification allows for extensive experimental manipulation and long-term studies on CD4+ T cell functions and mechanisms. Researchers can utilize this cell line to investigate various aspects of immune regulation, including T cell activation, cytokine production, and interactions with other immune cells, providing valuable insights into immune system diseases and potential therapeutic targets. Donor\/background information provided for this product: Male, 28, Caucasian.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal initiation of healthy cell cultures, we recommend using a 24-well plate. Once established, cells can be expanded in PriCoat™ T25 Flasks (G299) for continued growth. PrimGrow T Xpan™ Medium Kit (TM182) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. T Xpan™ Cell Activator (CD3\/CD28\/CD2) (TM139) is required for T cell activation upon thawing. Note 1: refer to the detailed \"Thawing Protocol\" and \"Subculturing Protocol\" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. Note 2: The post-thaw viability of cells can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in 24-well plate and activate before transferring to other culture vessels. Always supplement with fresh reagents. Note 3: We recommend cryopreserving early-passage activated cells as a backup stock for your experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Serial passaging and retroviral transduction with retroviruses carrying the SV40 gene.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180542157165,"sku":"T0417","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NF9lDumcykHjBzDOoEe3ZK240m07oCSC3dBGVEzi.png?v=1774957863","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-peripheral-blood-cd4-positive-helper-t-cell-line-negative-sv40-bhc10902170","provider":"BioHippo","version":"1.0","type":"link"}