{"product_id":"immortalized-human-prostate-stromal-cells-pm151t-negative-htert-bhc10900678","title":"Immortalized Human Prostate Stromal Cells (PM151T) - hTERT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eProstate cancer research has been hindered by the fact that the cell types involved in the process of malignant transformation have proven difficult to develop into continuously growing cultures. Stromal smooth muscle cells established in primary culture after radical prostatectomy from a 62-year-old patient were subjected to retroviral infection with human telomerase catalytic subunit (hTERT), thus creating Immortalized Human Prostate Stromal Cells (PM151T) - hTERT.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Prostate Stromal Cells (PM151T) - hTERT is supplied as a tumor cell line derived from Human prostate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. MCDB 131 medium (Sigma) + 15% decalcified horse serum (Sigma) + 10 mM pH 7.2 HEPES + 1X MEM Non-essential Amino Acid Solution + 5 µg\/ml insulin (TM053) + 10 µg\/ml transferrin (Sigma) + 10 nM estradiol + 100 nM dexamethasone (Sigma) + 5 ng\/ml sodium selenite (Sigma) + 1 ng\/ml basic FGF (Z101456) + 0.1 ng\/ml EGF (Z100139) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cells were able to proliferate for at least 190 days, express genes associated with smooth muscle function (progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin and myocardin) and exhibit up-regulation for some of these markers as well as contractile behavior upon induction of differentiation. The generation of this cell line by way of a method that minimizes complex alterations results in a faithful in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy. Characterization by Western blot, qRT-PCR, and transforming growth factor-β\/endothelin A treatment. Donor\/background information provided for this product: Male, 62.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. MCDB 131 medium (Sigma) + 15% decalcified horse serum (Sigma) + 10 mM pH 7.2 HEPES + 1X MEM Non-essential Amino Acid Solution + 5 µg\/ml insulin (TM053) + 10 µg\/ml transferrin (Sigma) + 10 nM estradiol + 100 nM dexamethasone (Sigma) + 5 ng\/ml sodium selenite (Sigma) + 1 ng\/ml basic FGF (Z101456) + 0.1 ng\/ml EGF (Z100139) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 2,500\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180505686381,"sku":"T0727","price":566.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-prostate-stromal-cells-pm151t-negative-htert-bhc10900678","provider":"BioHippo","version":"1.0","type":"link"}