{"product_id":"immortalized-human-proximal-tubule-cells-hpct-05wt-bhc10900676","title":"Immortalized Human Proximal Tubule Cells (HPCT-05wt)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eWater and electrolyte homeostasis is largely regulated by the human proximal tubule; however this complex system has been hard to study particularly in relation to specific genes and their influence on renal physiology and pathophysiology. Immortalized Human Proximal Convoluted Tubule Cells (HPCT-05wt) is a potential model to acquire detailed physiological information about cell behaviour in response to hormones, growth factors, nutrients, and other external stimuli.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Proximal Tubule Cells (HPCT-05wt) is supplied as an immortalized cell line derived from Human kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-cobblestone\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Cells are grown on Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 μm (Millipore) that are lightly coated with a 20% dispersion of rat-tail collagen (in 60% ethanol) plus 1 μg\/mL laminin. PriGrow IV (TM004) + 5% FBS +15 mM HEPES + 20 nM sodium selenite (Sigma) + 50 mM ascorbic acid-2-phosphate (Sigma) + 5 µg\/ml transferrin (Sigma) + 5 µg\/ml Insulin Solution (TM053) + 10 ng\/mL mouse EGF (Z100139) + 4 µg\/ml dexamethasone + 1 nM T3 (Sigma) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in kidney biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCharacterization by Western blot. Donor\/background information provided for this product: Male, 50.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Cells are grown on Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 μm (Millipore) that are lightly coated with a 20% dispersion of rat-tail collagen (in 60% ethanol) plus 1 μg\/mL laminin. PriGrow IV (TM004) + 5% FBS +15 mM HEPES + 20 nM sodium selenite (Sigma) + 50 mM ascorbic acid-2-phosphate (Sigma) + 5 µg\/ml transferrin (Sigma) + 5 µg\/ml Insulin Solution (TM053) + 10 ng\/mL mouse EGF (Z100139) + 4 µg\/ml dexamethasone + 1 nM T3 (Sigma) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 150,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180505948525,"sku":"T0747","price":679.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/s0sjYKpfvFQApvv35B1RK2heZ8nfXiknobzDbknp.png?v=1774957766","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-proximal-tubule-cells-hpct-05wt-bhc10900676","provider":"BioHippo","version":"1.0","type":"link"}