{"product_id":"immortalized-human-skeletal-muscle-cells-skm-1-bhc10900661","title":"Immortalized Human Skeletal Muscle Cells (SKM-1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Human Skeletal Muscle Cells (SKM-1) is immortalized via the introduction of only the cytoplasmic content (non-DNA) of tumor cells into primary human skeletal muscle cells thereby resulting in permanent growth activation. One key aspect of such immortalization method is its resemblance of the immortalized cells to its primary counterparts (i.e. grows in monolayer).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Skeletal Muscle Cells (SKM-1) is supplied as an immortalized cell line derived from Human skeletal muscle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, spindle-shaped\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow IV (TM004) + 5% FBS + 10 ng\/ml rhFGF2 (Z101455) + 10 ng\/ml GH1 (Z100265) + 10 ng\/ml rhHGF (Z102785) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells are thin and elongated. If seeded in large culture vessels, cells will take additional time to appear confluent as they pack closely.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in skeletal muscle biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eSKM-1 cells can be used for studying many aspects of muscular function and disease. Characterization conducted by IHC. Donor\/background information provided for this product: Not disclosed..\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow IV (TM004) + 5% FBS + 10 ng\/ml rhFGF2 (Z101455) + 10 ng\/ml GH1 (Z100265) + 10 ng\/ml rhHGF (Z102785) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells are thin and elongated. If seeded in large culture vessels, cells will take additional time to appear confluent as they pack closely.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000 - 40,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180504703341,"sku":"T0782","price":1617.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LOlmW2aPSc269LnZh5h7kaCtVO63XYDdJXWyQEOs.jpg?v=1774957767","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-skeletal-muscle-cells-skm-1-bhc10900661","provider":"BioHippo","version":"1.0","type":"link"}