{"product_id":"immortalized-human-skeletal-myoblasts-lhcn-m2-bhc10900659","title":"Immortalized Human Skeletal Myoblasts (LHCN-M2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eLHCN-M2 cell line, an immortalized human skeletal myoblasts from satellite cells of the pectoralis major muscle, engineered to maintain the fundamental features of primary human muscle progenitors while offering the scalability and consistency required for research.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Skeletal Myoblasts (LHCN-M2) is supplied as an immortalized cell line derived from Human skeletal muscle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Mix 4:1 PriGrow III (TM003) and PriGrow I (TM001) + 15% HI-FBS + 1.4 μg\/mL vitamin B12 + 0.055 μg\/mL dexamethasone + 0.03 μg\/mL zinc sulfate heptahydrate + 0.02 M HEPES (TM058) + 10 ng\/mL FGF2 (Z101455) + 2.5 ng\/mL human hepatocyte growth factor (rhHGF) (Z102785) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in skeletal muscle biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in skeletal muscle biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male, 41, Caucasian, Pectoralis.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Mix 4:1 PriGrow III (TM003) and PriGrow I (TM001) + 15% HI-FBS + 1.4 μg\/mL vitamin B12 + 0.055 μg\/mL dexamethasone + 0.03 μg\/mL zinc sulfate heptahydrate + 0.02 M HEPES (TM058) + 10 ng\/mL FGF2 (Z101455) + 2.5 ng\/mL human hepatocyte growth factor (rhHGF) (Z102785) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 3,125 - 5,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180505358701,"sku":"T0756","price":1555.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/xniuQPOdVZY9I0Iyg7wh04xf5yhoG8ZQQ0MpGnyM.png?v=1774957764","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-skeletal-myoblasts-lhcn-m2-bhc10900659","provider":"BioHippo","version":"1.0","type":"link"}