{"product_id":"immortalized-human-t-cells-bhc10900698","title":"Immortalized Human T Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Human T Cells are genetically modified to proliferate indefinitely while retaining their functional characteristics, making them an invaluable tool for immunological research and therapeutic development. These cells offer a consistent and reliable model for studying T cell biology, including mechanisms of activation, signaling pathways, and immune responses.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human T Cells is supplied as an immortalized cell line derived from Human blood.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round; cells grow as round clumps\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal initiation of healthy cell cultures, we recommend using a 24-well plate. Once established, cells can be expanded in PriCoat™ T25 Flasks (G299) for continued growth. PrimGrow T Xpan™ Medium Kit (TM182) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. T Xpan™ Cell Activator (CD3\/CD28\/CD2) (TM139) is required for T cell activation upon thawing. Note 1: refer to the detailed \"Thawing Protocol\" and \"Subculturing Protocol\" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. Note 2: The post-thaw viability of cells can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in 24-well plate and activate before transferring to other culture vessels. Always supplement with fresh reagents. Note 3: We recommend cryopreserving early-passage activated cells as a backup stock for your experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Transduction with Lenti-hTERT (LV616) lentivirus and Lenti-Rb siRNA (LV621) lentivirus.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eAdditionally, they are widely used in preclinical studies for developing and testing novel immunotherapies, including CAR-T cell therapies and vaccines. Their ability to be expanded in vitro without senescence allows for large-scale production and in-depth functional assays, facilitating advancements in personalized medicine and immunotherapy research. Donor\/background information provided for this product: Healthy donor, no other details disclosed.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal initiation of healthy cell cultures, we recommend using a 24-well plate. Once established, cells can be expanded in PriCoat™ T25 Flasks (G299) for continued growth. PrimGrow T Xpan™ Medium Kit (TM182) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. T Xpan™ Cell Activator (CD3\/CD28\/CD2) (TM139) is required for T cell activation upon thawing. Note 1: refer to the detailed \"Thawing Protocol\" and \"Subculturing Protocol\" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. Note 2: The post-thaw viability of cells can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in 24-well plate and activate before transferring to other culture vessels. Always supplement with fresh reagents. Note 3: We recommend cryopreserving early-passage activated cells as a backup stock for your experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Transduction with Lenti-hTERT (LV616) lentivirus and Lenti-Rb siRNA (LV621) lentivirus.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180506145133,"sku":"T0086","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t0086-abm.png?v=1774957769","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-t-cells-bhc10900698","provider":"BioHippo","version":"1.0","type":"link"}