{"product_id":"immortalized-mink-endometrial-epithelial-cells-gmme1-bhc10900720","title":"Immortalized Mink Endometrial Epithelial Cells (GMme1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eResearch on the phenomenon of obligate embryonic diapause in mustelids has been limited by largely unsuccessful attempts to maintain such embryos in culture. Immortalized Mink Endometrial Epithelial Cells (GMme1) were generated by stable transfection of endometrial cells isolated from mink uterine tissue with SV40 large T antigen.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mink Endometrial Epithelial Cells (GMme1) is supplied as an immortalized cell line derived from Mink endometrium.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Mix 1:1 PriGrow III (TM003) and F10 (Gibco, 11550043) + 5% FBS + 5% Nu serum + 10⁻⁷ M beta estradiol + 10 µg\/ml insulin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Immortalized with a plasmid vector containing the SV40 large T antigen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in endometrium biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cells maintain an epithelial morphology after numerous passages in culture, stain strongly for cytokeratin (immunocytochemistry) and, most intriguingly, enhance embryonic survival when employed in coculture. GMme1 therefore represents an important step towards establishing an in vitro model approximating the in vivo environment of the mink uterus. Expression information reported for the model: Cytokeratin expression, neomycin resistance (100 mg\/ml).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Mix 1:1 PriGrow III (TM003) and F10 (Gibco, 11550043) + 5% FBS + 5% Nu serum + 10⁻⁷ M beta estradiol + 10 µg\/ml insulin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Immortalized with a plasmid vector containing the SV40 large T antigen\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180506866029,"sku":"T0734","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bYgK4YbD7PP9yc9JrBIQrCG8t7qLlNZ4pKsC3A3n.png?v=1774957769","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mink-endometrial-epithelial-cells-gmme1-bhc10900720","provider":"BioHippo","version":"1.0","type":"link"}