{"product_id":"immortalized-mouse-conditionally-reprogrammed-otic-stem-cells-cr-osc-bhc10900737","title":"Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eOtic Stem Cells are auditory progenitor cells which can be differentiated into cells which resemble auditory hair cells (HC) and express HC genes. Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC) were immortalized through exposure to conditional reprogramming conditions and their growth rate remained consistent for 10 passages.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC) is supplied as an immortalized cell line derived from Mouse ear.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Grow cells in 6-well plates coated with mitomycin c (10 ug\/mL, 3 hours) inactivated 3T3-J2 cells (6 x 105 cells\/well) with the following conditions. PriGrow III (TM003) + 5% FBS + 0.4 ug\/mL hydrocortisone + 5 ug\/mL Insulin Solution (TM053) + 8.4 ng\/mL cholera toxin + 10 ng\/mL epidermal growth factor + 24 ug\/mL adenine + 5 uM Y-27632 (ROCK inhibitor) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cells upregulate HC genes in response to differentiation conditions, regardless of passage number. The Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC) are useful in the field of audiology. The applications of this cell line include monitoring and manipulating HC specific genes and experiments involving genetic mutations, knock-outs or overexpression, both of which are enhanced by the large number of cells this cell line produces. Can be differentiated into cells which resemble auditory hair cells (HC) and express HC genes. Donor\/background information provided for this product: Balb\/c Mouse, Organ of Corti.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Grow cells in 6-well plates coated with mitomycin c (10 ug\/mL, 3 hours) inactivated 3T3-J2 cells (6 x 105 cells\/well) with the following conditions. PriGrow III (TM003) + 5% FBS + 0.4 ug\/mL hydrocortisone + 5 ug\/mL Insulin Solution (TM053) + 8.4 ng\/mL cholera toxin + 10 ng\/mL epidermal growth factor + 24 ug\/mL adenine + 5 uM Y-27632 (ROCK inhibitor) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 400,000 - 800,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180507586925,"sku":"T0372","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6LcNLZFSFSxAQFe7A7z4IojioczQWwiiISU0BEZt.png?v=1774957773","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-conditionally-reprogrammed-otic-stem-cells-cr-osc-bhc10900737","provider":"BioHippo","version":"1.0","type":"link"}