{"product_id":"immortalized-mouse-dendritic-cells-mutudc1940-bhc10900734","title":"Immortalized Mouse Dendritic Cells (MutuDC1940)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eAs messengers bridging the innate and adaptive immune systems, conventional\/classical lymphoid tissue-resident dendritic cells (cDCs) capture and present antigens; acting as sentinels in secondary lymphoid organs and other tissues. The wild-type MutuDC1940 dendritic cell line is derived from mouse spleen tissues (CD11c:SV40LgT transgenic mice) and is a powerful tool for vaccine science and immunotherapy, particularly for strategizing target antigens to the CD8α+ subset (which has the CD11chigh, B220−, DEC205+, CD24high, CD11b− expression profile).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Dendritic Cells (MutuDC1940) is supplied as an engineered cell line derived from Mouse spleen.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, small aggregates\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 4 mM Glutamax + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES (TM058) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Filter the complete media with a 0.22µm filter prior to use.Note: cells must be seeded and maintained at high density for optimal growth. Cells are sensitive to culture conditions; change the complete media every 2-3 days. Do not let media colour change to yellow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Gfp reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eMutuDC cell lines are GFP positive due to the GFP reporter in the CD11c:SV40LgT transgene. T3034 - TLR3 Knockout MutuDC Cell line Cat. T3035 - TLR9 Knockout MutuDC Cell line Cat. T3036 - Ifnar1 Knockout MutuDC Cell line Donor\/background information provided for this product: CD11c:SV40LgT-transgenic C57BL\/6 mice.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 4 mM Glutamax + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES (TM058) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Filter the complete media with a 0.22µm filter prior to use.Note: cells must be seeded and maintained at high density for optimal growth. Cells are sensitive to culture conditions; change the complete media every 2-3 days. Do not let media colour change to yellow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e3D Culture Conditions:\u003c\/strong\u003e Procedure Preparation (before 3D Culture): Thaw cells following \"Thawing Protocol\" and \"Growth Conditions\". Expand cells for one passage, then seed for spheroid generation (3D culture). Subculture Protocol:Aspirate the supernatant and wash cells with 1X PBS (pH 7.4) (G5000). Dissociate cells using 1–1.5 ml of Gentle Dissociation Solution (TM080). Observe the cells under a microscope to confirm detachment (typically within 2–10 minutes). Cells that are difficult to detach can be put in 37 °C for several minutes to facilitate detachment.Neutralize using an equal volume of complete growth media (must contain serum) or using Trypsin Neutralizing Solution (TM069).Transfer culture suspension into sterile conical centrifuge tube (G5500), and centrifuge at 125 x g for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.Aspirate supernatant and re-suspend the pellet with pre-warmed fresh complete growth media for 3D Culture. Spheroid Generation Protocol:Count cells and viability using cell counter or hemocytometer with Trypan Blue Stain (TM071). Proceed if cells are \u0026gt;90% viability.Seed 500 to 10,000 cells per well in SpheroWell™ 96 Well Plate (G7540), final volume maintained at 100 μl per well. Avoid cell clumps (clumps will not generate uniform spheroids). Count as Day 0. Tip: Fill the outermost wells of the plate with 1X PBS (pH 7.4) (G5000) to prevent evaporation of media during incubation.(Recommended) Centrifuge the sealed plate at 300 x g for 5 minutes.Place in the incubator set at 37 °C, 5% CO₂. Change Media:Every 2–3 days, add 100 μl of fresh media slowly along the wall of the well to avoid disrupting spheroids at the bottom.Take out 100 μl of media slowly and discard from the well.(Recommended, but optional) Centrifuge the sealed plate at 300 x g for 5 minutes. Monitor the Cells for 3D\/Spheroid Formation:Spheroid formation will appear between day 5 and 10.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180507554157,"sku":"T0528","price":1390.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pKYxGYLGCl8dk6rLdFTHXVRy3cJGq1RN9ixml4GW.png?v=1774957771","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-dendritic-cells-mutudc1940-bhc10900734","provider":"BioHippo","version":"1.0","type":"link"}