{"product_id":"immortalized-mouse-enteric-neuronal-cells-bhc10900769","title":"Immortalized Mouse Enteric Neuronal Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Mouse Enteric Neuronal Cells were isolated from a transgenic mouse with a mutation of the SV-40 tsA58 gene. These novel cells are very stable- biologically and phenotypically- for multiple generations, in vitro.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Enteric Neuronal Cells is supplied as an immortalized cell line derived from Mouse intestine.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 52 ng\/ml selenium (Sigma) + 16.11 µg\/ml putrescine (Sigma) + 5 µg\/ml Insulin (TM053) + 1 µg\/ml transferrin (Sigma) + 0.1 mg\/ml fetuin (Sigma, Cat No. F3385) + 1 µg\/ml BSA (Bio Basic) + 6.3 ng\/ml progesterone (Sigma) + 2 mM L-Glutamine (G275). Filter solution with 0.45 µM before adding 10% FBS* (Regular) + 10 ng\/ml mouse IFN-gamma (Z200085) + 100 ng\/ml human GDNF (Z101055) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThus, they are perfectly suited for research focusing on the enteric nervous system; from determining the impact of disease on the enteric nervous system to simply tracking its development. Neuronal markers were assessed and confirmed via western blot and immunocytochemical analysis. Donor\/background information provided for this product: H-2Kb-tsA58 Transgenic Mice.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 52 ng\/ml selenium (Sigma) + 16.11 µg\/ml putrescine (Sigma) + 5 µg\/ml Insulin (TM053) + 1 µg\/ml transferrin (Sigma) + 0.1 mg\/ml fetuin (Sigma, Cat No. F3385) + 1 µg\/ml BSA (Bio Basic) + 6.3 ng\/ml progesterone (Sigma) + 2 mM L-Glutamine (G275). Filter solution with 0.45 µM before adding 10% FBS* (Regular) + 10 ng\/ml mouse IFN-gamma (Z200085) + 100 ng\/ml human GDNF (Z101055) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000 - 50,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180508602733,"sku":"T0297","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GHDSFpvLsvtz5TIXoQYw5yl3M2lBlRJfWrKIHmoG.png?v=1774957778","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-enteric-neuronal-cells-bhc10900769","provider":"BioHippo","version":"1.0","type":"link"}