{"product_id":"immortalized-mouse-epidermal-keratinocytes-cdc25a-f7-bhc10900768","title":"Immortalized Mouse Epidermal Keratinocytes (CDC25A F7)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eCell division cycle 25 (CDC25) family of phosphatases consists of CDC25A, CDC25B and CDC25C in which regulates the cell cycle progression, and thus are important for the activation of cell cycle checkpoints in response to DNA damage. CDC25A degradation has been associated with cell cycle arrest.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) is supplied as an immortalized cell line derived from Mouse skin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobble-stone\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Thaw the cells with PriGrow XI (TM011) + 8% FBS + 60 µM CaCl₂ (added fresh each time) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ for optimal attachment. After 24h, change media to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng\/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ Steps to thaw the cells: 1. Thaw the cells in RT water until only a few ice crystals are left 2. Put the cells in 5 ml of RT PriGrow XI (TM011) + 8% FBS 3. Centrifuge the cells 1500 rpm for 5 min (need this step to remove inhibitors for attachment) 4. Resuspend the pellet in RT Prigrow XI (TM011) + 8% FBS + 1% P\/S with freshly added 60 µM CaCl₂ and put the cells in a culture vessel at 37.0°C, 8% CO₂ 5. After 18h-24h, change to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng\/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ for growth of the cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in skin biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eImmortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells were isolated from the Cdc25afl\/fl\/Cre- mice. The cells are suitable as a control for the mutant cells, in which CDC25A is not expressed, Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells (Cat. No. T0298). Donor\/background information provided for this product: C57\/BL6 Cdc25afl\/fl\/Cre- mouse.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Thaw the cells with PriGrow XI (TM011) + 8% FBS + 60 µM CaCl₂ (added fresh each time) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ for optimal attachment. After 24h, change media to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng\/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ Steps to thaw the cells: 1. Thaw the cells in RT water until only a few ice crystals are left 2. Put the cells in 5 ml of RT PriGrow XI (TM011) + 8% FBS 3. Centrifuge the cells 1500 rpm for 5 min (need this step to remove inhibitors for attachment) 4. Resuspend the pellet in RT Prigrow XI (TM011) + 8% FBS + 1% P\/S with freshly added 60 µM CaCl₂ and put the cells in a culture vessel at 37.0°C, 8% CO₂ 5. After 18h-24h, change to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng\/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 8% CO₂ for growth of the cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180507652461,"sku":"T0299","price":2080.6,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-epidermal-keratinocytes-cdc25a-f7-bhc10900768","provider":"BioHippo","version":"1.0","type":"link"}