{"product_id":"immortalized-mouse-lung-clara-cells-negative-conditionally-c22-bhc10900799","title":"Immortalized Mouse Lung Clara Cells - Conditionally (C22)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eClara cells play a role in the maintenance of normal bronchiolar epithelium by secreting Clara Cell Secretory Proteins (i.e. CC10) to protect the respiratory tract against oxidative stress and inflammation.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Lung Clara Cells - Conditionally (C22) is supplied as an immortalized cell line derived from Mouse lung.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, spindle-shaped\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 100 U\/ml rmIFNG (Z200085) + 5 ug\/ml transferrin + 25 ng\/ml rmEGF (Z200025) + 10 ug\/ml insulin (Z101065) + 7.5 µg\/ml Endothelial Cell Growth Supplement (TM130) + 0.25 ug\/ml endothelin-1 + 20 ng\/ml T3 + 0.36 ug\/ml hydrocortisone + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂.Please note: the optimal incubator temperature for cell proliferation is 33.0°C.For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in lung biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe C22 is a conditionally immortalized mouse lung clara cells isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kb promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. C22 resembles closely to primary clara cells and constitutively expresses and secretes surfactant proteins SP-A, SP-B, SP-D and CC10. This cell line is useful in studying lung epithelial physiology, as well as studying the role of CC10 in pulmonary inflammation and viral infection.Presence of CC10, SP-A, SP-B and absence of SP-C confirmed by RT-PCR. Donor\/background information provided for this product: H-2Kb-tsA58 transgenic mouse.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 100 U\/ml rmIFNG (Z200085) + 5 ug\/ml transferrin + 25 ng\/ml rmEGF (Z200025) + 10 ug\/ml insulin (Z101065) + 7.5 µg\/ml Endothelial Cell Growth Supplement (TM130) + 0.25 ug\/ml endothelin-1 + 20 ng\/ml T3 + 0.36 ug\/ml hydrocortisone + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂.Please note: the optimal incubator temperature for cell proliferation is 33.0°C.For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180509061485,"sku":"T0092","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T0092_Morphology.png?v=1774957776","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-lung-clara-cells-negative-conditionally-c22-bhc10900799","provider":"BioHippo","version":"1.0","type":"link"}