{"product_id":"immortalized-mouse-lung-endothelial-cells-negative-pmt-bhc10900798","title":"Immortalized Mouse Lung Endothelial Cells - PmT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003ePrimary mouse lung endothelial cells were derived and immortalized with polyoma middle T (PmT) virus to generate the Immortalized Mouse Lung Endothelial Cells – PmT. The cells are valuable for downstream applications to study the lung endothelium, as they can continuously divide in culture.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Lung Endothelial Cells - PmT is supplied as an immortalized cell line derived from Mouse lung.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\/round-shaped\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Cells must be grown in T25 Flasks (G999) pre-coated with Gelatin Coating Solution (TM063) supplemented with Applied Cell Extracellular Matrix (G422, 0.3%) and Fibronectin coating solution (TM059, 10µg\/ml). Mix 1:1 ratio of Prigrow IV (TM004) and PriGrow VI (TM016) + 10% heat inactivated FBS + 2mM L-Glutamine (G275) + 100µg\/mL Heparin + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: this cell line is slow growing and requires ECGS for growth. Cells take additional time to attach (7-10 days) and proliferate. Do not change media during this window; additional complete media can be added to the culture vessel during this time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Immortalized via infection with polyoma middle T (PmT) virus.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line may serve as a useful model for studies relating to diseases and understanding the biology of lung endothelial cells.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Cells must be grown in T25 Flasks (G999) pre-coated with Gelatin Coating Solution (TM063) supplemented with Applied Cell Extracellular Matrix (G422, 0.3%) and Fibronectin coating solution (TM059, 10µg\/ml). Mix 1:1 ratio of Prigrow IV (TM004) and PriGrow VI (TM016) + 10% heat inactivated FBS + 2mM L-Glutamine (G275) + 100µg\/mL Heparin + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: this cell line is slow growing and requires ECGS for growth. Cells take additional time to attach (7-10 days) and proliferate. Do not change media during this window; additional complete media can be added to the culture vessel during this time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Immortalized via infection with polyoma middle T (PmT) virus.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180508832109,"sku":"T0094","price":597.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t0094.png?v=1774957775","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-lung-endothelial-cells-negative-pmt-bhc10900798","provider":"BioHippo","version":"1.0","type":"link"}