{"product_id":"immortalized-mouse-microglia-cells-eoc-2-bhc10902256","title":"Immortalized Mouse Microglia Cells (EOC 2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eEOC 2 cells are a microglial cell line derived from a murine model. These cells are commonly used in neuroinflammation and neurodegenerative disease research.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Microglia Cells (EOC 2) is supplied as an immortalized cell line derived from Mouse brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, macrophage\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Alternative media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 4 mM L-Glutamine (G275) + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEOC 2 microglia cells retain key characteristics of primary microglia, such as immune response activation and cytokine production, making them valuable for studying neuroinflammatory processes, brain injury, and neurodegenerative conditions like Alzheimer's and Parkinson's disease. Donor\/background information provided for this product: Female, C3H\/HeJ 10 Day Mouse, Microglia.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Alternative media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 4 mM L-Glutamine (G275) + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2 or 1:4\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180544549229,"sku":"T1225","price":710.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/M1MZ11qzYPNWu0aWf2pzfz8t7fQu5v2vnqq94FzO.png?v=1774957867","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-microglia-cells-eoc-2-bhc10902256","provider":"BioHippo","version":"1.0","type":"link"}