{"product_id":"immortalized-mouse-preadipocyte-cell-line-bhc10000005","title":"Immortalized Mouse Preadipocyte Cell Line","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#0f5d57;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #15706A}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e6f2f0;color:#0f5d57;font-weight:600;vertical-align:top;border:1px solid #cfe3e0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #cfe3e0}\n.bhp-desc .bhp-comp-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}\n.bhp-desc .bhp-comp-table th{padding:8px 10px;background:#15706A;color:#fff;font-weight:600;text-align:left;border:1px solid #cfe3e0}\n.bhp-desc .bhp-comp-table td{padding:8px 10px;vertical-align:top;border:1px solid #cfe3e0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#e6f2f0;border:1px solid #cfe3e0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#0f5d57;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#15706A;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCell Lines\u003c\/span\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eAdipose tissue exists in two functionally distinct forms. Brown adipose tissue (BAT) is the body's primary site of non-shivering thermogenesis, packed with UCP-1–rich mitochondria, while white adipose tissue (WAT) is the principal energy-storage depot and an active endocrine organ secreting adipokines. Immortalized preadipocyte lines from both depots — at young and aged donor stages — provide a matched in vitro panel for studies of adipogenesis, thermogenesis, lipid metabolism, mitochondrial biology, and the metabolic decline associated with aging.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThis product is a panel of four immortalized mouse preadipocyte cell lines, selectable by \u003cstrong\u003eCell Type\u003c\/strong\u003e (brown or white) and \u003cstrong\u003eDonor Age\u003c\/strong\u003e (young or aged). All lines were derived from C57BL\/6 mouse stromal vascular fraction cells, immortalized by retroviral delivery of the SV40 large T-antigen, and enriched by neomycin (G418) selection. They proliferate stably and differentiate into mature adipocytes — UCP-1–positive brown adipocytes or PPARγ+\/FABP4+ white adipocytes — making the set ideal for comparative adipocyte aging and metabolism research. Select your line using the options above.\u003c\/p\u003e\n\u003ch2\u003eVariant Comparison\u003c\/h2\u003e\n\u003ctable class=\"bhp-comp-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eLine (SKU)\u003c\/th\u003e\n\u003cth\u003eTissue \/ Depot\u003c\/th\u003e\n\u003cth\u003eDonor Age\u003c\/th\u003e\n\u003cth\u003eStrain\u003c\/th\u003e\n\u003cth\u003eDifferentiation marker\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrown, Young (BHC10000001)\u003c\/td\u003e\n\u003ctd\u003eInterscapular brown adipose tissue (iBAT)\u003c\/td\u003e\n\u003ctd\u003e1 day (neonatal)\u003c\/td\u003e\n\u003ctd\u003eC57BL\/6J\u003c\/td\u003e\n\u003ctd\u003eUCP-1–positive brown adipocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrown, Old (BHC10000002)\u003c\/td\u003e\n\u003ctd\u003eInterscapular brown adipose tissue (iBAT)\u003c\/td\u003e\n\u003ctd\u003e27 months (aged)\u003c\/td\u003e\n\u003ctd\u003eC57BL\/6J\u003c\/td\u003e\n\u003ctd\u003eUCP-1–positive brown adipocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWhite, Young (BHC10000003)\u003c\/td\u003e\n\u003ctd\u003eSubcutaneous white adipose tissue (sWAT)\u003c\/td\u003e\n\u003ctd\u003eYoung\u003c\/td\u003e\n\u003ctd\u003eC57BL\/6\u003c\/td\u003e\n\u003ctd\u003ePPARγ+\/FABP4+ white adipocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWhite, Old (BHC10000004)\u003c\/td\u003e\n\u003ctd\u003eSubcutaneous white adipose tissue (sWAT)\u003c\/td\u003e\n\u003ctd\u003e18 months (aged)\u003c\/td\u003e\n\u003ctd\u003eC57BL\/6J\u003c\/td\u003e\n\u003ctd\u003ePPARγ+\/FABP4+ white adipocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eShared Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eMouse (\u003cem\u003eMus musculus\u003c\/em\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStrain\u003c\/th\u003e\n\u003ctd\u003eC57BL\/6 \/ C57BL\/6J\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSex\u003c\/th\u003e\n\u003ctd\u003eMale\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Type\u003c\/th\u003e\n\u003ctd\u003ePreadipocyte (brown or white, by variant)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmortalization\u003c\/th\u003e\n\u003ctd\u003eSV40 large T-antigen (retroviral); neomycin\/G418 selectable marker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGrowth Properties\u003c\/th\u003e\n\u003ctd\u003eAdherent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiosafety Level\u003c\/th\u003e\n\u003ctd\u003eBSL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eCryovial, 1.0 ml (~2 × 10\u003csup\u003e5\u003c\/sup\u003e cells)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eLiquid nitrogen (≤ –160 °C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eQuality Control \u0026amp; Validation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eSterility: Pass\u003c\/span\u003e \u003cspan class=\"bhp-qc-badge\"\u003eMycoplasma: Pass\u003c\/span\u003e \u003cspan class=\"bhp-qc-badge\"\u003ePost-freeze viability ~80%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003ePassage number varies by line (3–5). Recovery: seed into a T25 flask at 2 × 10\u003csup\u003e5\u003c\/sup\u003e cells; reaches ~90% confluence within ~4 days. A lot-specific Certificate of Analysis is provided with each vial (Certificates for the brown young, brown aged, and white aged lines are attached under Documents \/ References).\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eComplete growth medium:\u003c\/strong\u003e DMEM\/F-12 supplemented with 10% fetal bovine serum (FBS) and 100 µg\/ml Primocin. Filter-sterilize through a 0.2 µm filter; store up to 6 months at 4 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrowth \u0026amp; culture conditions:\u003c\/strong\u003e Maintain at 37 °C in a humidified 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator. Refresh growth medium every other day. Subculture at 80–90% confluence using a 1:3–1:4 split ratio.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSubculture \/ propagation:\u003c\/strong\u003e Aspirate medium and wash once with 1× PBS. Add 0.25% trypsin-EDTA to cover the cell surface and incubate at 37 °C for 2–3 min until cells round up. Neutralize with growth medium, collect, and centrifuge for 3 min at 400 × g. Resuspend and reseed at a 1:3–1:4 ratio.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThawing:\u003c\/strong\u003e Thaw the cryovial in a 37 °C water bath for 2–3 min. Centrifuge 3 min at 400 × g (4 °C) and remove the freezing medium. Resuspend in growth medium and seed to reach ~40–50% confluence. Culture at 37 °C, 5% CO\u003csub\u003e2\u003c\/sub\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCryopreservation medium:\u003c\/strong\u003e 90% FBS and 10% DMSO (filter-sterilized). Store in liquid nitrogen for long-term preservation.\u003c\/p\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eSafety \u0026amp; Handling:\u003c\/strong\u003e Biosafety Level 2 (BSL-2). Handle in a certified biosafety cabinet following institutional guidelines for primary mammalian cells. Wear appropriate PPE and decontaminate all waste before disposal.\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eResearch Use\u003c\/strong\u003eFor Research Use Only (RUO)\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBiosafety\u003c\/strong\u003eBSL-2\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eSpecies\u003c\/strong\u003eMouse (C57BL\/6)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures. This product is sold for laboratory research purposes; it is the buyer's responsibility to ensure compliance with applicable institutional and governmental regulations.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Biohippo Inc","offers":[{"title":"Brown \/ Young \/ 1 cryovial (~2 × 10^5 cells)","offer_id":53485460062573,"sku":"BHC10000001","price":650.0,"currency_code":"USD","in_stock":true},{"title":"Brown \/ Old \/ 1 cryovial (~2 × 10^5 cells)","offer_id":53485460095341,"sku":"BHC10000002","price":650.0,"currency_code":"USD","in_stock":true},{"title":"White \/ Young \/ 1 cryovial (~2 × 10^5 cells)","offer_id":53485460128109,"sku":"BHC10000003","price":650.0,"currency_code":"USD","in_stock":true},{"title":"White \/ Old \/ 1 cryovial (~2 × 10^5 cells)","offer_id":53485460160877,"sku":"BHC10000004","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fig2_brown_morphology_6dda8b58-2247-4135-8780-dfabc364a045.png?v=1782413766","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-preadipocyte-cell-line-bhc10000005","provider":"BioHippo","version":"1.0","type":"link"}