{"product_id":"immortalized-mouse-promyelocyte-cells-mpro-c-2-negative-sv40-bhc10900815","title":"Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 is a immortalized cell line supplied in frozen format with defined immortalization\/background engineering and associated with Mouse bone marrow biology.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 is supplied as an immortalized cell line derived from Mouse bone marrow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round; may form aggregates\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 20% Horse Serum + 25 ng\/ml mouse IL-3 (Z200445) + 2.5 ng\/ml Recombinant Mouse GM-CSF (CSF2) (Z200075) + 10ng\/ml Recombinant Human IL1B (Z100395) + 20ng\/ml Recombinant Human IL6 (Z100555) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: allow cells to recover during the first 24-48 hours post-thaw.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Immortalized with retroviral vector LRARα403SN.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in immunology, hematology, and signaling. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male, 6 weeks. Expression information reported for the model: Express high levels of retroviral mRNA harboring the truncated RARα403 sequence, mouse neutrophil-specific antigen 7, positive staining for chloroacetate esterase.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 20% Horse Serum + 25 ng\/ml mouse IL-3 (Z200445) + 2.5 ng\/ml Recombinant Mouse GM-CSF (CSF2) (Z200075) + 10ng\/ml Recombinant Human IL1B (Z100395) + 20ng\/ml Recombinant Human IL6 (Z100555) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: allow cells to recover during the first 24-48 hours post-thaw.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Immortalized with retroviral vector LRARα403SN.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180509487469,"sku":"T0686","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/tYQFDNkjj8Sg0Cw69D27lWIqQQtQ953XJbkE0Sbw.png?v=1774957776","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-promyelocyte-cells-mpro-c-2-negative-sv40-bhc10900815","provider":"BioHippo","version":"1.0","type":"link"}