{"product_id":"immortalized-mouse-proximal-caput-epididymal-epithelial-cell-line-pc1-bhc10900813","title":"Immortalized Mouse Proximal Caput Epididymal Epithelial Cell Line (PC1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003ePC1 is a conditionally immortalized mouse epididymal epithelial cell line isolated from the proximal caput of the mouse harbouring a temperature-sensitive simian virus 40 large T antigen. The epididymal epithelium plays an important role in male fertility by supporting sperm motility, maturation and viability.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Proximal Caput Epididymal Epithelial Cell Line (PC1) is supplied as an immortalized cell line derived from Mouse testes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in testes biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eOwing to the changing microenvironment created by different sections of the epididymis, the spermatozoa matures as they passage through the convoluted tubule. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro at permissive temperature (33°C). At non-permissive temperatures (37°C-39°C), the cells cease to proliferate. PC1 expresses androgen receptor as well as markers of the murine epididymal epithelium, PEB-like protein (i.e. phosphatidylethanolamine binding protein), E-RABP (i.e. epididymal retinoic acid-binding protein), and EP17 (i.e. epididymal protein of 17 kd). Together with the Immortalized Mouse Distal Caput Epididymal Epithelial Cell Line (DC2) (Cat. No. T0599), these cells are useful in studying the regulation of tissue-specific gene expression and epididymal specific proteins in the epididymis. Immunocytochemistry, western blot and RT-PCR were used to confirm expression of markers.Additional cell lines from this panel:Cat. T0599 - Immortalized Mouse Distal Caput Epididymal Epithelial Cell Line (DC2)Note: both cell lines are from the caput of the epididymis but differ in their region of isolation within the caput (proximal vs distal). Donor\/background information provided for this product: 10-12 weeks old tsSV40 transgenic mouse, Caput epididymis.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30,000 - 50,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180509946221,"sku":"T0598","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/qDd1YYntk4pzicZEA4awzbuFA4DGzQUoIlhU2YfB.png?v=1774957778","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-proximal-caput-epididymal-epithelial-cell-line-pc1-bhc10900813","provider":"BioHippo","version":"1.0","type":"link"}