{"product_id":"immortalized-mouse-subcutaneous-adipose-multipotent-mesenchymal-cells-m17-asc-bhc10900849","title":"Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eA spontaneously immortalized cell clone arisen from isolated adipose-derived stem cells (ASCs), the Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) display a typical fibroblastoid morphology. The cell line stably expresses the stem cell markers Sca-1, c-Kit\/CD117, nestin, nucleostemin, CD44, and CD106, but is negative for the embryo stem cell markers Nanog, Oct4, and Sox2.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) is supplied as an immortalized cell line derived from Mouse adipose.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Claycomb medium (Sigma) + 2 mM L-glutamine (G275) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cells were endowed with multipotency and can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. It displays a normal karyotype and stable telomeres, neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD\/SCID-g null mice. The cell line is thus recommended as a valuable tool for a number of applications in ex vivo tissue engineering to study the differentiation mechanisms involved in tissue repair, as well as a model for both pharmacological and toxicological studies. RT-PCR and Cytofluorimetry were performed on the cDNA from the cells to confirm the expression of stem cell markers. Expression information reported for the model: Stem cell markers (Sca-1, c-kit, Islet 1, nestin, and nucleostemin), CD44, CD106,.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Claycomb medium (Sigma) + 2 mM L-glutamine (G275) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180511813997,"sku":"T0542","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/jl4iCA2egb8A2X49ZM3sRn0SHTNJ2JuRJ2BocJpB.png?v=1774957782","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-subcutaneous-adipose-multipotent-mesenchymal-cells-m17-asc-bhc10900849","provider":"BioHippo","version":"1.0","type":"link"}