{"product_id":"immortalized-mouse-tlr4-minus-minus-hepatic-stellate-cells-bhc10900846","title":"Immortalized Mouse TLR4 -\/- Hepatic Stellate Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Mouse TLR4⁻\/⁻ Hepatic Stellate Cells were derived from TLR4 knockout mice, generated via targeted disruption of the Tlr4 gene in embryonic stem cells using electroporation of a linearized targeting construct. Primary hepatic stellate cells were isolated by enzymatic digestion and Percoll density gradient centrifugation, then immortalized via transfection of the pCMV Simian Virus 40 Large T antigen with hygromycin B selection.As TLR4 is a key receptor in pro-inflammatory signaling, these cells exhibit impaired NF-κB activation in response to LPS, making them ideal for studies of liver fibrosis, scar tissue formation, and apoptosis resistance linked to TLR4 signaling.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse TLR4 -\/- Hepatic Stellate Cells is supplied as an immortalized cell line derived from Mouse liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Transfection of pCMV Simian Virus 40 Large T antigen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in liver biology, metabolism, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cells have also been successfully used in TLR4 lentivirus-mediated knock-in experiments using Plenti4\/TO\/V5-DEST Gateway™ vectors (Invitrogen) and Lipofectamine™ 2000 (Thermo Fisher Scientific), enabling mechanistic studies of TLR4-dependent survival and inflammatory pathways. Donor\/background information provided for this product: C57\/Bl6 WT mouse.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Transfection of pCMV Simian Virus 40 Large T antigen\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180510994797,"sku":"T0689","price":731.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MXrkpJbRAy53AsJNdpVgxo3Zr4I1ON69Jh6VTCz4.png?v=1774957779","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-tlr4-minus-minus-hepatic-stellate-cells-bhc10900846","provider":"BioHippo","version":"1.0","type":"link"}