{"product_id":"immortalized-mouse-tumour-dendritic-cells-cd4-mutudc2-bhc10900843","title":"Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2) was isolated from the spleen of Batf3-\/- CD11c-SV40LgT transgenic mice in C57BL\/6JOlaHsd background. The CD4⁻ MutuDC2s display high levels of CD11c and MHC-II with lack of the pDC-specific markers B220, Gr-1 and PDCA-1.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2) is supplied as a tumor cell line derived from Mouse spleen.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, small aggregates\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. IMDM (1x) + Glutamax (Gibco, 31980-030) + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Filter the complete media at 0.22 µm before use\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Spontaneous SV40LgT-mediated tumorigenic transformation of splenic conventional Dendritic Cells in CD11c-SV40LgT-transgenic mice\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in spleen biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIn their resting state they are CD80hi and CD86hi. These observations demonstrate that the CD4⁻ MutuDC2s have a relatively mature splenic resident conventional DC1 phenotype. They can be easily manipulated with standard experimental techniques and display great stability of their phenotypic and functional properties in long term culture as long as their passage number is maintained below 40-50. For these reasons the CD4⁻ MutuDC2s represent a new valuable tool for the study of dendritic cell biology. Donor\/background information provided for this product: Batf3-\/- CD11c-SV40LgT transgenic mice in C57BL\/6JOlaHsd background.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. IMDM (1x) + Glutamax (Gibco, 31980-030) + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Filter the complete media at 0.22 µm before use\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Spontaneous SV40LgT-mediated tumorigenic transformation of splenic conventional Dendritic Cells in CD11c-SV40LgT-transgenic mice\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180510830957,"sku":"T0790","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/gQcGkx53UyzPj1XHWz00JtS4ImL2erprdeKmvLCS.png?v=1774957779","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-mouse-tumour-dendritic-cells-cd4-mutudc2-bhc10900843","provider":"BioHippo","version":"1.0","type":"link"}