{"product_id":"immortalized-myeloid-monocyte-cells-mgb8e-c57-cas9gfp-bhc10902194","title":"Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line an immortalized myeloid monocyte progenitor cell line made in the background of C57Bl\/6 Cas9-GFP transgenic mice. The cells express GFP at low concentrations detectable by flow cytometry (note: it can be impossible to see GFP expression by fluorescent microscopy). mGB8e-C57-Cas9GFP cells are transduced with ER-HoxB8.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP) is supplied as an engineered cell line derived from Mouse bone marrow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Half suspension\/ half adherent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture; passage to new plastic with fresh media at least every 4 days. The base medium for this cell line is PriGrow II (TM002) supplemented with 10% FBS + 10 ng\/mL GM-CSF (Z200075) + 0.5 μM ß-estradiol + 1% Penicillin\/Streptomycin (G255),37.0°C, 5% CO₂.ß-estradiol is required for cell maintenance but must be completely removed for cell differentiation.Cells are sensitive to post-thaw conditions but recover after 24 hours in culture.For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells can be differentiated into mature monocytes. This panel of cell lines is suitable for immunological research. Additional cell lines within this panel include: Cat. T0200 - Immortalized Myeloid Granulocyte Cell Line (ECoM-G) Cat. T0201 - Immortalized Myeloid Monocyte Cell Line (ECoM-M) Cat. T0202 - Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO) Cat. T0203 - Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO) Cat. T0204 - Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP) Cat. T0205 - Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP) Cat. T0207 - Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57) Cat. T0209 - Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture; passage to new plastic with fresh media at least every 4 days. The base medium for this cell line is PriGrow II (TM002) supplemented with 10% FBS + 10 ng\/mL GM-CSF (Z200075) + 0.5 μM ß-estradiol + 1% Penicillin\/Streptomycin (G255),37.0°C, 5% CO₂.ß-estradiol is required for cell maintenance but must be completely removed for cell differentiation.Cells are sensitive to post-thaw conditions but recover after 24 hours in culture.For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:10-1:20 every 3-4 days\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180542845293,"sku":"T0205","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/kMBbiBTLo8gGJsfGpqOzMYE8XwtloC7Ed6fnV3JW.png?v=1782151806","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-myeloid-monocyte-cells-mgb8e-c57-cas9gfp-bhc10902194","provider":"BioHippo","version":"1.0","type":"link"}