{"product_id":"immortalized-myeloid-monocyte-cells-mgb8e-c57stem-erko-bhc10902193","title":"Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThese cells are made in the background of the C57Bl\/6 CD45.1 congenic mouse strain (Mercier et al.) that was crossed with the estrogen-receptor knockout mouse strain (https:\/\/www.jax.org\/strain\/026176). The CD45.1 marker allows for easy in vivo tracking of the cells.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO) is supplied as an engineered cell line derived from Mouse bone marrow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Half suspension\/ half adherent , round\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture; passage to new plastic with fresh media at least every 4 days. The base medium for this cell line PriGrow II (TM002) supplemented with 10% FBS + 10 ng\/mL GM-CSF (Z200075) + 0.5 μM ß-estradiol + 1% Penicillin\/Streptomycin (G255),37.0°C, 5% CO₂. ß-estradiol is required for cell maintenance but must be completely removed for cell differentiation.Cells are sensitive to post-thaw conditions but recover after 24 hours in culture.For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe ER-KO was performed to control for any undesired effects of exogenous estradiol on the progenitor cell function. This panel of cell lines is suitable for immunological reasearch. Additional cell lines within this panel include: Cat. T0200 - Immortalized Myeloid Granulocyte Cell Line (ECoM-G) Cat. T0201 - Immortalized Myeloid Monocyte Cell Line (ECoM-M) Cat. T0202 - Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO) Cat. T0203 - Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO) Cat. T0204 - Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP) Cat. T0205 - Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP) Cat. T0207 - Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57) Cat. T0209 - Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture; passage to new plastic with fresh media at least every 4 days. The base medium for this cell line PriGrow II (TM002) supplemented with 10% FBS + 10 ng\/mL GM-CSF (Z200075) + 0.5 μM ß-estradiol + 1% Penicillin\/Streptomycin (G255),37.0°C, 5% CO₂. ß-estradiol is required for cell maintenance but must be completely removed for cell differentiation.Cells are sensitive to post-thaw conditions but recover after 24 hours in culture.For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:10-1:20 every 3-4 days\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180543172973,"sku":"T0203","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mmO9ZZbaUxsVGQSqia7pP0Esjbj1XHg6jO9s0njx.png?v=1782151804","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-myeloid-monocyte-cells-mgb8e-c57stem-erko-bhc10902193","provider":"BioHippo","version":"1.0","type":"link"}