{"product_id":"immortalized-nf1-cutaneous-neurofibroma-cell-line-icnf98-4c-negative-htert-bhc10901657","title":"Immortalized NF1 Cutaneous Neurofibroma Cell Line (icNF98.4c) - hTERT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003ePrimary Schwann cells, obtained from surgical patient donations, were transduced with retroviral vectors containing hTERT. These cell lines are useful for basic biology\/neuroscience investigations, tumor investigations, genetic manipulations or preclinical studies.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized NF1 Cutaneous Neurofibroma Cell Line (icNF98.4c) - hTERT is supplied as an engineered cell line derived from Human skin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Cell growth is enhanced with supplementation of 25 ng\/mL Neuregulin (Z101625). For optimal performance, subculture cells at high confluency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Transduction with lentiviruses carrying hTERT (pTYcPPT).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in neurobiology, differentiation, and cell signaling. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male, 60-70.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Cell growth is enhanced with supplementation of 25 ng\/mL Neuregulin (Z101625). For optimal performance, subculture cells at high confluency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Transduction with lentiviruses carrying hTERT (pTYcPPT).\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180532588909,"sku":"T0186","price":1627.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EEoV4xuSFLIUvPkLZPnedIxk9yemdwpxXfINyQE6.png?v=1782151800","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-nf1-cutaneous-neurofibroma-cell-line-icnf98-4c-negative-htert-bhc10901657","provider":"BioHippo","version":"1.0","type":"link"}