{"product_id":"immortalized-normal-chondrocytes-niac-htert-and-sv40lt-bhc10902121","title":"Immortalized Normal Chondrocytes (NiAC)- hTERT \u0026 SV40LT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis immortalized articular cartilage condrocyte (NiAC) cell line (Cat. T0384) is derived from normal (non-osteoarthritic) donor tissue.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Normal Chondrocytes (NiAC)- hTERT \u0026amp; SV40LT is supplied as an immortalized cell line derived from Human cartilage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIt can be used in combination with the immortalized articular cartilage condrocyte (OA iAC) (Cat. T0385) derived from an osteoarthritic patient as in vitro model of osteochondral regeneration and disease. The chondrocyte cell lines form three-dimensional aggregates when subjected to the hanging drop method. Furthermore, the aggregates present an extracellular matrix containing collagen.Complete list of cell lines from this panel: Cat. T0377 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 6Cat. T0378 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 8 Cat. T0379 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 9 Cat. T0380 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 10 Cat. T0381 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 12 Cat. T0382 - Immortalized Mesenchymal Stromal Cell Line (iMSC) - Clone 13 Cat. T0383 - Immortalized Normal Synoviocyte Cell Line (iSin) - hTERT \u0026amp; SV40LT Cat. T0384 - Immortalized Normal Chondrocytes (N iAC)- hTERT \u0026amp; SV40LT Cat. T0385 - Immortalized Osteoarthritic Chondrocytes (OA iAC)- hTERT \u0026amp; SV40LT Donor\/background information provided for this product: non-OA patient, Cartilage of femoral head.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 69.6\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180541600109,"sku":"T0384","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/oStN686w8pfOvWrNFlcFsHe5gMksdSsiLRiIkwlY.png?v=1782151798","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-normal-chondrocytes-niac-htert-and-sv40lt-bhc10902121","provider":"BioHippo","version":"1.0","type":"link"}