{"product_id":"immortalized-pck-cholangiocyte-cells-pck-ccl-bhc10900829","title":"Immortalized PCK Cholangiocyte Cells (PCK CCL)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized PCK Cholangiocyte Cell Line (PCK CCL) is a well-characterized epithelial cell model derived from the liver of the PCK rat, a widely used animal model of Autosomal Recessive Polycystic Kidney Disease (ARPKD). These cells retain key cholangiocyte features, including biliary epithelial morphology, polarization, and cyst-forming ability in 3D culture, making them an essential tool for studying cystogenesis and biliary pathophysiology.Developed and characterized by Muff et al. (2006), the PCK CCL line exhibits abnormal cellular proliferation and fluid secretion properties reflective of ARPKD pathology, while maintaining markers consistent with differentiated cholangiocytes.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized PCK Cholangiocyte Cells (PCK CCL) is supplied as an immortalized cell line derived from Rat liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow I (TM001) + 10% FBS (*Regular) + 2 mM L-glutamine (G275) + 1X NEAA (TM068) + 1X Chemically Defined Lipid Concentrate + 1X MEM Vitamin Solution + 1X ITS (TM052) + 50 µg\/ml Soybean Trypsin Inhibitor + 15 µg\/ml BPE + 25 ng\/ml EGF (Z100139) + 393 ng\/ml Dexamethasone + 3.4 µg\/ml T3 + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in liver biology, metabolism, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cells have been immortalized to ensure long-term in vitro utility and reproducibility across experiments. Donor\/background information provided for this product: Male, 3 month, PCK\/CrljCrl-Pkhd1pck\/Crl, Polycystic Kidney Disease (PCK) Rat.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow I (TM001) + 10% FBS (*Regular) + 2 mM L-glutamine (G275) + 1X NEAA (TM068) + 1X Chemically Defined Lipid Concentrate + 1X MEM Vitamin Solution + 1X ITS (TM052) + 50 µg\/ml Soybean Trypsin Inhibitor + 15 µg\/ml BPE + 25 ng\/ml EGF (Z100139) + 393 ng\/ml Dexamethasone + 3.4 µg\/ml T3 + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/ml):\u003c\/strong\u003e 300,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180510175597,"sku":"T0828","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/kg1FzeMFTyYPo8t4P6S66yuiWy7v33A8wfkPO64y.png?v=1774957778","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-pck-cholangiocyte-cells-pck-ccl-bhc10900829","provider":"BioHippo","version":"1.0","type":"link"}