{"product_id":"immortalized-podocyte-progenitor-cell-line-um51-pre-podo-htert-bhc10902205","title":"Immortalized Podocyte Progenitor Cell Line (UM51-Pre-Podo-hTERT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe UM51-Pre-Podo-hTERT cell line was derived from progenitor cells isolated from the urine of a 51-year-old African male and immortalized using hTERT. These cells have been shown to proliferate indefinitely while retaining key podocyte characteristics.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Podocyte Progenitor Cell Line (UM51-Pre-Podo-hTERT) is supplied as an immortalized cell line derived from Human kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobblestone-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of gelatin coating (TM063) is required for cell adhesion to the culture vessel and optimal proliferation. PriGrow X series medium (TM0208) + + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: cells display contact inhibition and should be subcultured when 70% confuence to maintain characteristics. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eUM51-Pre-Podo-hTERT cells express several podocyte-specific markers, such as WT1, NPHS1, and SYNPO which are crucial for understanding the differentiation and functional capacity of podocytes. Despite continuous culture, the UM51-Pre-Podo-hTERT line maintains a stable morphology, proliferation capacity, and expression of critical podocyte markers. It also displays contact inhibition and P53 expression, suggesting maintained normal cellular controls. This cell line has been differentiated into mature podocytes and can enter the G0 phase, thus making it suitable for studying podocyte maturation and function, as well as for modeling diseases where podocyte quiescence and re-entry into the cell cycle play a role. Overall, the UM51-hTERT-Pre-Podo line is an excellent model for research studying nephrogenesis and kidney-associated diseases. Donor\/background information provided for this product: 51, Male, African, Urine.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of gelatin coating (TM063) is required for cell adhesion to the culture vessel and optimal proliferation. PriGrow X series medium (TM0208) + + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: cells display contact inhibition and should be subcultured when 70% confuence to maintain characteristics. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:10\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180543926637,"sku":"T0208","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/X8beBCRrfaCLCfgwsjwBVNEqqkrwNjBUShdJbmMy.png?v=1774957864","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-podocyte-progenitor-cell-line-um51-pre-podo-htert-bhc10902205","provider":"BioHippo","version":"1.0","type":"link"}