{"product_id":"immortalized-porcine-fibroblast-cell-line-ikuf04-bhc10902172","title":"Immortalized Porcine Fibroblast Cell Line (iKUF04)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe iKUF04 cell line is derived from ear tissue of an NIH miniature pig and has a unique distinction of being homozygous across all tested SLA loci (SLA-1, SLA-2, SLA-3, DQA, DQB1, and DRB1), which is rare and immensely valuable for controlled immunological studies. This homozygosity makes it an ideal candidate for studying the effects of specific SLA alleles on immune responses.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Porcine Fibroblast Cell Line (iKUF04) is supplied as an immortalized cell line derived from Pig muscle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 2 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cell line has been immortalized with hTERT and cultured up to 30 passages, maintaining stable growth and characteristic fibroblast features.Additional cell lines from this panel: T0390 - Immortalized Porcine Fibroblast Cell Line (iKUF01)T0391 - Immortalized Porcine Fibroblast Cell Line (iKUF02) T0392 - Immortalized Porcine Fibroblast Cell Line (iKUF03) T0393 - Immortalized Porcine Fibroblast Cell Line (iKUF04) T0394 - Immortalized Porcine Fibroblast Cell Line (iKUF07) T0395 - Immortalized Porcine Fibroblast Cell Line (iKUF09) Donor\/background information provided for this product: NIH miniature pig.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 2 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 1,800\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180542910829,"sku":"T0393","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/u1Vy4sXrOv1CpvZUqyVPaEuFv56vMd64N4wnnasJ.jpg?v=1774957860","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-porcine-fibroblast-cell-line-ikuf04-bhc10902172","provider":"BioHippo","version":"1.0","type":"link"}