{"product_id":"immortalized-rat-brain-vascular-endothelial-cells-gpnt-bhc10900893","title":"Immortalized Rat Brain Vascular Endothelial Cells (GPNT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe blood-brain barrier protects the central nervous system from potentially harmful substances by controlling the passage of cells and molecules. This barrier consists of vascular tissue, without pores, where the endothelial cells form tight junctions that allow only for select molecules to pass via pinocytosis.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Brain Vascular Endothelial Cells (GPNT) is supplied as an immortalized cell line derived from Rat blood vessel.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Ham’s F10 Medium (Lonza) + heat-inactivated 10% FBS + 2 mM L-Glutamine (G275) + 2 ng\/ml rrFGF2 (Z300005) + 80 μg\/ml Heparin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eImmortalized Rat Brain Vascular Endothelial Cells (GPNT) was derived from microvessels in the cerebral cortex. The cells were immortalized by lipofection using an SV40 retroviral vector with a temperature sensitive large T antigen (tsA58) and contain a neomycin resistance gene. This cell line retains many characteristics of its primary parent cell line including expression of central nervous system specific markers, such as P-glycoprotein and GLUT-1, a high affinity glucose transporter not found in other vascular endothelial tissue. In addition, this cell line also expresses important immunological molecules including VCAM-I, PECAM-1 and MHC class I and II. Due to these properties this cell line may be useful in investigating immunological function, signal transduction, and migration of immune cells in specialized brain vascular endothelial tissue. More generally this cell line is ideal for research on the blood brain barrier. Donor\/background information provided for this product: Lewis rat.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Ham’s F10 Medium (Lonza) + heat-inactivated 10% FBS + 2 mM L-Glutamine (G275) + 2 ng\/ml rrFGF2 (Z300005) + 80 μg\/ml Heparin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30, 000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180511781229,"sku":"T0685","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6lCsESzHk97bvoDxxAzaG9aTWEU5tNNnuuqhTj6Z.png?v=1774957784","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-brain-vascular-endothelial-cells-gpnt-bhc10900893","provider":"BioHippo","version":"1.0","type":"link"}