{"product_id":"immortalized-rat-conjunctival-epithelial-cells-cj4-1a-negative-sv40-bhc10900889","title":"Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) - SV40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) were derived from primary conjunctival epithelial cells from Fischer 344 rats. The primary cells were immortalized with pSV(neo) and transfected with SV40 large T antigen.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) - SV40 is supplied as an immortalized cell line derived from Rat eye.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 0.5% BSA + 10mM HEPES (TM058) + X trace elements B + 10μg\/ml Insulin (TM053) + 5.5μg\/ml Transferrin + 50ng\/ml Sodium selenate + 20μg\/ml Ethanolamine + 0.3μg\/ml Glucagon + 0.1μM Dexamethasone + 0.1μM Retinoic acid + 0.3μM 3, 3’, 5 triiodo-L thyronine + 80ng\/ml EGF (Z300045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe immortalized cells display epithelial morphology and express the conjunctiva-specific cytokeratin 4, the goblet cell-specific cytokeratin 7 and were negative for the corneal epithelial cell-specific cytokeratin 12. The cells are useful as models for studying the conjunctival epithelial barrier and also in developing strategies for delivering macromolecular antigens across this barrier to underlying components of the immune system. Immunofluorescence confocal microscopy demonstrated cytokeratin expression. Donor\/background information provided for this product: Fischer 344 Rat.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 0.5% BSA + 10mM HEPES (TM058) + X trace elements B + 10μg\/ml Insulin (TM053) + 5.5μg\/ml Transferrin + 50ng\/ml Sodium selenate + 20μg\/ml Ethanolamine + 0.3μg\/ml Glucagon + 0.1μM Dexamethasone + 0.1μM Retinoic acid + 0.3μM 3, 3’, 5 triiodo-L thyronine + 80ng\/ml EGF (Z300045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 48 - 60\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180512043373,"sku":"T0368","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bfCsZyAO2fECKw7kiDhtaP1lWyCafo0i1Z44DKpV.png?v=1774957783","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-conjunctival-epithelial-cells-cj4-1a-negative-sv40-bhc10900889","provider":"BioHippo","version":"1.0","type":"link"}