{"product_id":"immortalized-rat-microglia-negative-sv40-bhc10900924","title":"Immortalized Rat Microglia - SV40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Rat Microglia – SV40 cell line is derived from primary rat microglial cells and immortalized using SV40. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.This immortalization strategy enables the cells to maintain long-term proliferative capacity while retaining key microglial characteristics, making them a valuable model for neuroinflammation, neurodegeneration, and CNS immune response research.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Microglia - SV40 is supplied as an immortalized cell line derived from Rat brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Poly-L-Lysine Coating Solution (TM061) coated flasks are required for optimal cell culture. PriGrow IV (TM004) + 10% FBS (*Regular) + 2 mM L-Glutamine (G275) + 20 ng\/ml mouse GM-CSF (Z200075) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Due to cell size, the cells may appear more confluent than they appear. Morphology may change when media becomes acidic, but usually reverts back to normal morpholohgy once media is changed. Recommend to change media with fresh mouse GM-CSF every 2-3 days. Keeping the cells at a high density is also recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in neurobiology, differentiation, and cell signaling. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Poly-L-Lysine Coating Solution (TM061) coated flasks are required for optimal cell culture. PriGrow IV (TM004) + 10% FBS (*Regular) + 2 mM L-Glutamine (G275) + 20 ng\/ml mouse GM-CSF (Z200075) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Due to cell size, the cells may appear more confluent than they appear. Morphology may change when media becomes acidic, but usually reverts back to normal morpholohgy once media is changed. Recommend to change media with fresh mouse GM-CSF every 2-3 days. Keeping the cells at a high density is also recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2 - 1:5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30, 000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180512600429,"sku":"T0246","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/P62m5kPJ28lKya3fPuEjsTHFTM3q19mFI0IUvUtK.png?v=1774957784","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-microglia-negative-sv40-bhc10900924","provider":"BioHippo","version":"1.0","type":"link"}