{"product_id":"immortalized-rat-neural-stem-cells-1464r-bhc10900922","title":"Immortalized Rat Neural Stem Cells (1464R)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Rat Neural Stem Cells (1464R) are spontaneously immortal population of neural stem cells derived from the brain stem. The cells retained morphology even after 50 passages.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Neural Stem Cells (1464R) is supplied as an immortalized cell line derived from Rat brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA, #P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA) + 10 ng\/mL of FGF2 (Z101455) + 10 ng\/mL of EGF (Z100139) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIn the presence of retinoic acid, the cells may differentiate to TuJ1-positive neurons and to GFAP-positive astrocytes or O4-positive oligodendrocytes. May be used for brain disease studies, and a source for neuronal and glial cells. Donor\/background information provided for this product: 12 weeks old.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA, #P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA) + 10 ng\/mL of FGF2 (Z101455) + 10 ng\/mL of EGF (Z100139) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. For information regarding cell differentiation, please refer to the \"Differentiation Protocol\" PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Spontaneous immortalization\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180513452397,"sku":"T0746","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/5YaAMBAorKL73DRyuFdbdpEJOJxBLcWXCBS42BjH.png?v=1774957786","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-neural-stem-cells-1464r-bhc10900922","provider":"BioHippo","version":"1.0","type":"link"}