{"product_id":"immortalized-rat-ovarian-granulosa-cells-rga-1-bhc10900920","title":"Immortalized Rat Ovarian Granulosa Cells (RGA-1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eOvarian granulosa cells play an important physiological role in supporting the development and selection of ovarian follicles by controlling oocyte maturation and by producing the steroid hormones estradiol and progesterone; which are critical for maintenance of the ovarian cycle. The immortalized rat granulosa cells exhibit a temperature-dependent phenotype with respect to morphology as well as growth and the expression of prohibitin.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Ovarian Granulosa Cells (RGA-1) is supplied as an immortalized cell line derived from Rat ovary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Loosely adherent and suspension\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 2.5% FBS + 10 µg\/ml low-density lipoprotein + 30 µg\/ml high-density lipoprotein + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Immortalized with a temperature-sensitive mutant (tsA209) of SV40\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eRGA-1 cells will serve as a useful model in studies related to the functional relevance of prohibitin during cell differentiation and apoptosis. Donor\/background information provided for this product: Female, 27 days old.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 2.5% FBS + 10 µg\/ml low-density lipoprotein + 30 µg\/ml high-density lipoprotein + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Immortalized with a temperature-sensitive mutant (tsA209) of SV40\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180512567661,"sku":"T0758","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t0758-fig1.jpg?v=1774957785","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-ovarian-granulosa-cells-rga-1-bhc10900920","provider":"BioHippo","version":"1.0","type":"link"}