{"product_id":"immortalized-rat-retinal-pigment-epithelial-cells-bpei-1-bhc10900910","title":"Immortalized Rat Retinal Pigment Epithelial Cells (BPEI-1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eRetinal pigment epithelial (RPE) cells perform a variety of roles in the support of photoreceptor function, but are not amenable to in vitro analysis, as a tedious dissection process yields relatively sparse and slow-growing primary cultures of finite life span. Immortalized Rat Retinal Pigment Epithelial Cells (BPEI-1) arose spontaneously from primary culture as isolated colonies that continued to proliferate indefinitely through successive passages.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rat Retinal Pigment Epithelial Cells (BPEI-1) is supplied as an immortalized cell line derived from Rat eye.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 20% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in eye biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cells exhibit hallmarks of transformation, can assume either a fibroblastic or epitethelioid form under different serum conditions, are immunoreactive for the RPE markers cytokeratin and cellular retinaldehyde binding protein and, like primary cells, avidly phagocytize outer photoreceptor rod segments. Given that pathology in RPE cells can lead to degeneration of photoreceptors and has been implicated in several retinal disorders, a continuous line such as BPEI-1 has obvious value in facilitating any number of studies that might be otherwise impractical with primary cultures. Characterization conducted by immunofluorescence localization and phagocytosis assay. Donor\/background information provided for this product: 7 days old.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 20% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 18\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180512108909,"sku":"T0731","price":494.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/7hSGxEaClUIJe2dTsYO0Bf1YGZVCdGjWRP0d57uU.png?v=1774957784","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rat-retinal-pigment-epithelial-cells-bpei-1-bhc10900910","provider":"BioHippo","version":"1.0","type":"link"}