{"product_id":"immortalized-rett-mouse-ear-tip-fibroblast-cells-ietf-mecp2-wt-bhc10900938","title":"Immortalized Rett Mouse Ear Tip Fibroblast Cells (iETF-Mecp2-WT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Rhett Mouse Fibroblast Cells (iETF-Mecp2-WT) may used alongside with Immortalized Rhett mouse fibroblast cell line with R255X mutation (iETF-Mecp2-R255X, T0870) and\/or Immortalized Rhett mouse fibroblast cell line with R294X mutation (iETF-Mecp2-R294X, T0871) to study the effects of the mutations. Fibroblast cells were derived from mouse ear pinna tissue and were transfected with with a plasmid encoding the SV40 large T antigen (pBSSVD2005).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Rett Mouse Ear Tip Fibroblast Cells (iETF-Mecp2-WT) is supplied as an immortalized cell line derived from Mouse ear.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% heat-inactivated FBS + 1mM Sodium Pyruvate (TM057) + 10mM HEPES (TM058) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in ear biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in ear biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Adult C57BL\/6J mouse, Rett syndrome.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% heat-inactivated FBS + 1mM Sodium Pyruvate (TM057) + 10mM HEPES (TM058) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 1,500 - 1,700\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180513190253,"sku":"T0869","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t0869_morphology.png?v=1774957785","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-rett-mouse-ear-tip-fibroblast-cells-ietf-mecp2-wt-bhc10900938","provider":"BioHippo","version":"1.0","type":"link"}