{"product_id":"immortalized-slc4a11-positive-mouse-corneal-endothelial-cells-bhc10900935","title":"Immortalized Slc4a11+\/+ Mouse Corneal Endothelial Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Slc4a11+\/+ Mouse Corneal Endothelial Cells were isolated from the cornea of progeny mice produced by the intercrossing of Slc4a11þ\/þ and Slc4a11\/C57BL\/6 mice with H-2Kb-tsA58 Immortomouse, which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen. Immortalized Slc4a11+\/+ Mouse Corneal Endothelial Cells also demonstrates temperature sensitive and IFN-c dependent proliferation.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Slc4a11+\/+ Mouse Corneal Endothelial Cells is supplied as an immortalized cell line derived from Mouse eye.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow I (TM001) + 8% Heat-inactivated FBS + 0.08% chondroitin sulfate (Sigma) + 200 μg\/ml CaCl2 (Sigma) + 100 μg\/ml pituitary extract (Biomedical Technologies. Inc.) + 5 ng\/ml EGF (Z200025) + 5.22 ng\/ml IFN-γ (Z200085) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Intercrossed the Slc4a11þ\/þ and Slc4a11\/C57BL\/6 mice with H-2Kb-tsA58 Immortomouse, which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line represents a useful model for the investigation of pathology and therapies for SLC4A11 associated congenital hereditary endothelial dystrophy (CHED) and Fuchs’ endothelial corneal dystrophy. Growing cells at 37.0°C will decrease tsTAg levels and stop proliferation.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow I (TM001) + 8% Heat-inactivated FBS + 0.08% chondroitin sulfate (Sigma) + 200 μg\/ml CaCl2 (Sigma) + 100 μg\/ml pituitary extract (Biomedical Technologies. Inc.) + 5 ng\/ml EGF (Z200025) + 5.22 ng\/ml IFN-γ (Z200085) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 19\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Intercrossed the Slc4a11þ\/þ and Slc4a11\/C57BL\/6 mice with H-2Kb-tsA58 Immortomouse, which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180512862573,"sku":"T0698","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/g5G3nYluldJili6ClxXq7u8WCeApIafO21vhxzlQ.png?v=1782151796","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-slc4a11-positive-mouse-corneal-endothelial-cells-bhc10900935","provider":"BioHippo","version":"1.0","type":"link"}