{"product_id":"immortalized-sweat-gland-myoepithelial-cell-line-iem-bhc10900933","title":"Immortalized Sweat Gland Myoepithelial Cell Line (iEM)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis novel, immortalized ecrine sweat gland myoepithelial cell line (iEM) serves as a model for research on sweat glands and sweating. These cells were developed from primary cells transduced with lentiviral vectors containing hTERT and SV40Tt.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Sweat Gland Myoepithelial Cell Line (iEM) is supplied as an engineered cell line derived from Human sweat gland.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round spheroid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids. Mammocult Human Medium Kit (Stemcell Technologies, Cat. 05620) + 10 ng\/ml EGF (Z100139) + 10 ng\/ml bFGF (Z101456) + 4 µg\/ml heparin + 0.5 µg\/ml hydrocortisone 21-hemisuccinate + 2% (v\/v) growth factor reduced matrigel (optional) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase. Grow cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form. Subculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells\/well.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in sweat gland biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells display spheroid formation with increased population doubling compard to non-transduced cells. iEM's express myoepithelial cell-specific proteins (a-SMA, B1 or K14) and can differentiate into luminal cells. Immortalized Sweat Gland Myoepithelial Cells provide a useful in vitro model for recapitulating eccrine sweat gland functions and for screening substances that can modulate sweating by acting on sweat glands. These cells are applicable for studies in hyperhidrosis, adiaphoresis, and sweat gland diseases. Please note: cells are exected to grow to 65 population doublings (PDs), however, the exact PD limit can vary by lot. Donor\/background information provided for this product: Female, 53, Eyelid.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids. Mammocult Human Medium Kit (Stemcell Technologies, Cat. 05620) + 10 ng\/ml EGF (Z100139) + 10 ng\/ml bFGF (Z101456) + 4 µg\/ml heparin + 0.5 µg\/ml hydrocortisone 21-hemisuccinate + 2% (v\/v) growth factor reduced matrigel (optional) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase. Grow cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form. Subculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells\/well.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 24 - 46\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180513223021,"sku":"T0816","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ETJSDY3IzW3GuvcEny2cJZwYxtG8auMvZ0JkE2La.png?v=1774957786","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-sweat-gland-myoepithelial-cell-line-iem-bhc10900933","provider":"BioHippo","version":"1.0","type":"link"}