{"product_id":"impa1-antibody-inositol-monophosphatase-1-bha17136189","title":"IMPA1 Antibody \/ Inositol monophosphatase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIMPA1 Antibody \/ Inositol monophosphatase 1 is a anti-IMPA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IMPA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIMPA1 antibody recognizes Inositol monophosphatase 1, a magnesium-dependent enzyme that catalyzes the dephosphorylation of inositol monophosphate to free inositol, a crucial step in the phosphatidylinositol signaling pathway. The IMPA1 gene encodes a cytosolic enzyme essential for recycling inositol phosphates derived from the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). This reaction is critical for maintaining inositol supply for the synthesis of phosphoinositides, which regulate intracellular signaling, neurotransmission, and osmoregulation.\u003cbr\u003e\u003cbr\u003eIMPA1 is expressed abundantly in brain, kidney, and liver, reflecting its central role in inositol metabolism. In neurons, IMPA1 supports the phosphoinositide cycle, enabling sustained neurotransmitter signaling via inositol trisphosphate and diacylglycerol pathways. Pharmacologically, IMPA1 is a well-known molecular target of lithium, a mood-stabilizing drug used in the treatment of bipolar disorder. Lithium inhibits IMPA1 activity, leading to inositol depletion and modulation of neuronal signaling, supporting the �inositol depletion hypothesis� for mood stabilization.\u003cbr\u003e\u003cbr\u003eThe human IMPA1 gene is located on chromosome 8q21.13 and encodes a 277-amino acid protein that functions as a homodimer. The enzyme binds magnesium ions within its active site to hydrolyze phosphate from a range of inositol monophosphate substrates. Structural studies have revealed conserved residues critical for catalysis and lithium binding. Genetic or functional alterations in IMPA1 have been associated with neurological disorders, cognitive deficits, and certain metabolic diseases. Experimental evidence also implicates IMPA1 in maintaining cellular osmotic balance, particularly in renal epithelial cells where inositol acts as a compatible osmolyte.\u003cbr\u003e\u003cbr\u003eImmunohistochemical analysis using IMPA1 antibody shows strong cytoplasmic staining in neurons, glial cells, renal tubules, and hepatocytes. The antibody is useful for studying inositol phosphate metabolism, lithium pharmacology, and neuronal signaling pathways. IMPA1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IMPA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IMPA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IMPA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IMPA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IMPA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321035117,"sku":"FY13287","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b9b7d272-643d-4060-8b0d-4777344595e9.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/impa1-antibody-inositol-monophosphatase-1-bha17136189","provider":"BioHippo","version":"1.0","type":"link"}