{"product_id":"inpp5f-antibody-type-ii-inositol-1-4-5-trisphosphate-5-phosphatase-bha17135611","title":"INPP5F Antibody \/ Type II inositol 1,4,5-trisphosphate 5-phosphatase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eINPP5F Antibody \/ Type II inositol 1,4,5-trisphosphate 5-phosphatase is a anti-INPP5F Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e INPP5F\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eINPP5F antibody detects Type II inositol 1,4,5-trisphosphate 5-phosphatase (also known as phosphatidylinositol polyphosphate 5-phosphatase INPP5F), an enzyme that regulates phosphoinositide metabolism and intracellular signaling. Encoded by the INPP5F gene on chromosome 10q26.11, this protein hydrolyzes the 5-phosphate group from phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate, thereby modulating PI3K\/AKT and phosphatidylinositol signaling pathways. By controlling local phosphoinositide concentrations, INPP5F regulates membrane dynamics, vesicle trafficking, and cardiac hypertrophy signaling. It acts as a negative regulator of PI3K-dependent cell growth and survival, contributing to cellular homeostasis and organ size control.\u003cbr\u003e\u003cbr\u003eINPP5F localizes to the cytoplasm and Golgi apparatus and is expressed in multiple tissues, with highest levels in heart, brain, and skeletal muscle. Functional studies have demonstrated that INPP5F attenuates insulin and growth factor signaling by dephosphorylating PIP3, thereby reducing AKT activation. In cardiomyocytes, INPP5F serves as an intrinsic brake on hypertrophic signaling by modulating the PI3K\/AKT\/mTOR axis. Mice lacking INPP5F display enhanced cardiac growth and increased susceptibility to heart failure, underscoring its physiological importance in maintaining normal cardiac morphology and function.\u003cbr\u003e\u003cbr\u003eThe INPP5F antibody is widely used in signal transduction, cardiovascular, and metabolic research to study phosphoinositide regulation and AKT signaling. Western blot analysis typically reveals a 108 kilodalton band corresponding to the full-length enzyme. Immunofluorescence shows perinuclear and Golgi-associated staining patterns consistent with its subcellular localization. Because INPP5F interacts with lipid-binding proteins and signaling kinases, this antibody is also employed in co-immunoprecipitation and pathway-mapping assays. Reduced INPP5F expression has been observed in glioblastoma, prostate cancer, and other tumors, suggesting its function as a tumor suppressor through attenuation of PI3K-driven oncogenic pathways.\u003cbr\u003e\u003cbr\u003eIn addition to its phosphatase activity, INPP5F contributes to endosomal sorting and receptor recycling by shaping local phosphoinositide pools on endomembranes. It cooperates with SHIP2 and synaptojanin to balance phosphoinositide turnover in response to receptor activation. The INPP5F antibody enables detailed examination of these signaling processes and provides insight into how lipid signaling defects contribute to disease.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=INPP5F - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=INPP5F - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=INPP5F - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/INPP5F - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=INPP5F+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297671533,"sku":"FY12708","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_26e190dd-22d9-42f3-89ac-f2452ad8fe4b.jpg?v=1782237034","url":"https:\/\/www.ebiohippo.com\/products\/inpp5f-antibody-type-ii-inositol-1-4-5-trisphosphate-5-phosphatase-bha17135611","provider":"BioHippo","version":"1.0","type":"link"}