{"product_id":"insulin-antibody-bha17103853","title":"Insulin Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eInsulin antibody supplied as a ascites reagent for IHC-P in Human, Rabbit, Rat samples. This product is a monoclonal (mouse origin) antibody (host: Mouse; isotype: Mouse IgG1) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Monoclonal (mouse origin); host Mouse; isotype Mouse IgG1; clone KC36AC10.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Ascites; purity: Ascites.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human, Rabbit, Rat.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e IHC-P.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e Human insulin was used as the immunogen for this Insulin antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eInsulin is the intended antigen for this primary antibody. Reported biological context includes: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of two dissimilar polypeptide chains, A and B, which are linked by two disulfide bonds. The gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - NCBI Gene search (Insulin) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Insulin - Ensembl search (Insulin) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Insulin - PubMed search (Insulin) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Insulin - Reactome pathway search (Insulin) — Reactome — https:\/\/reactome.org\/content\/query?q=Insulin --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS \/ 100 ug","offer_id":53043251708269,"sku":"R30045","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b3a03afa-935c-41a8-9c8c-d2933957fd85.jpg?v=1771934430","url":"https:\/\/www.ebiohippo.com\/products\/insulin-antibody-bha17103853","provider":"BioHippo","version":"1.0","type":"link"}