{"product_id":"interleukin-1-beta-antibody-il1b-bha17136363","title":"Interleukin 1 beta Antibody \/ IL1B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eInterleukin 1 beta Antibody \/ IL1B is a anti-IL1B Rabbit antibody Recombinant Rabbit Monoclonal clone 28I10 supplied in Purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IL1B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 28I10, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eInterleukin 1 beta antibody is a central tool for investigating inflammation, innate immunity, and chronic disease. The encoded protein, IL1B, is a proinflammatory cytokine primarily produced by activated macrophages, monocytes, and dendritic cells. It is first synthesized as an inactive proprotein that requires cleavage by caspase 1 within the inflammasome complex to generate active interleukin 1 beta. Once secreted, it binds to the interleukin 1 receptor type I, initiating signaling pathways that drive fever, leukocyte recruitment, and proinflammatory gene expression.\u003cbr\u003e\u003cbr\u003eIL1B is a master regulator of immune responses. It promotes the release of secondary mediators including tumor necrosis factor alpha and interleukin 6, amplifying inflammatory cascades. By activating NF kappa B and MAP kinase pathways, interleukin 1 beta induces transcription of genes involved in cell survival, proliferation, and differentiation. In adaptive immunity, IL1B shapes T helper cell polarization and antibody production, linking innate signals to downstream immune functions.\u003cbr\u003e\u003cbr\u003eAberrant interleukin 1 beta signaling is associated with many diseases. Excessive production contributes to rheumatoid arthritis, inflammatory bowel disease, psoriasis, and gout. Elevated IL1B levels are also observed in cardiovascular disease, metabolic disorders such as type 2 diabetes, and neurodegenerative diseases where chronic inflammation worsens tissue injury. Because of its pathogenic potential, IL1B is a major therapeutic target, and several inhibitors have been developed to block its activity in clinical settings.\u003cbr\u003e\u003cbr\u003eAt the molecular level, IL1B exerts its effects by binding to interleukin 1 receptor type I, which recruits the receptor accessory protein IL1RAcP to form a signaling complex. This complex engages adaptors such as MyD88 and kinases like IRAK, culminating in NF kappa B and AP 1 activation. These transcription factors coordinate expression of inflammatory genes, ensuring rapid and sustained immune responses.\u003cbr\u003e\u003cbr\u003eThe Interleukin 1 beta antibody is used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect both pro and mature forms of IL1B. These applications are essential for studying inflammasome activation, cytokine regulation, and the pathology of inflammatory disease. For researchers examining innate immunity, therapeutic blockade of cytokines, or chronic inflammation, the Interleukin 1 beta antibody is a reliable and specific reagent.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IL1B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IL1B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IL1B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IL1B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IL1B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047326933357,"sku":"RQ8945","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_43e2bde3-731e-4bd6-ba9b-65fb7f57277e.jpg?v=1772019448","url":"https:\/\/www.ebiohippo.com\/products\/interleukin-1-beta-antibody-il1b-bha17136363","provider":"BioHippo","version":"1.0","type":"link"}