{"product_id":"iqce-antibody-iq-domain-containing-protein-e-bha17135374","title":"IQCE Antibody \/ IQ domain-containing protein E","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIQCE Antibody \/ IQ domain-containing protein E is a anti-IQCE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IQCE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIQCE antibody recognizes IQ domain-containing protein E, a calcium-regulated component essential for the assembly and stability of primary cilia. IQCE belongs to a family of IQ motif-containing proteins that bind calmodulin and function in calcium signaling regulation. Within ciliated cells, IQCE localizes at the basal body and transition zone, helping to anchor structural and signaling complexes necessary for ciliogenesis. The IQCE antibody is a critical reagent for exploring developmental biology, signaling regulation, and ciliopathies caused by defective ciliary assembly.\u003cbr\u003e\u003cbr\u003eIQCE is encoded by the IQCE gene located on human chromosome 7p22.3. The protein contains one or more IQ motifs, short consensus sequences that mediate calcium-calmodulin interactions. These motifs enable IQCE to sense calcium changes and respond by altering its binding partners and structural conformation. It interacts with EF-hand calcium-binding protein EFCAB1 to form a complex required for the stability of the ciliary base. This partnership regulates Hedgehog signaling, a pathway central to embryogenesis and tissue differentiation. Disruption of IQCE causes ciliopathies, including acrocallosal syndrome, characterized by brain malformation, craniofacial defects, and polydactyly.\u003cbr\u003e\u003cbr\u003eWith the IQCE antibody, western blot analysis typically reveals a 77 kilodalton band, while immunofluorescence shows punctate staining at centrosomes and basal bodies. Ciliary localization is confirmed through co-staining with gamma-tubulin and acetylated tubulin. Loss-of-function studies demonstrate that IQCE depletion shortens or eliminates primary cilia, disrupting Hedgehog target gene activation. The protein also supports the recruitment of intraflagellar transport (IFT) components, ensuring proper axonemal elongation.\u003cbr\u003e\u003cbr\u003eBeyond ciliary biology, IQCE expression in epithelial and fibroblast cells suggests additional roles in vesicular trafficking, cytoskeletal organization, and mechanosensory signaling. Its calcium sensitivity allows IQCE to integrate mechanical and biochemical cues, influencing organ development and tissue maintenance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IQCE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IQCE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IQCE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IQCE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IQCE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289348461,"sku":"FY12471","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9bc2491d-01b0-451b-9f06-22f2e7acb72a.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/iqce-antibody-iq-domain-containing-protein-e-bha17135374","provider":"BioHippo","version":"1.0","type":"link"}