{"product_id":"iqcm-antibody-iq-motif-containing-protein-m-bha17135754","title":"IQCM Antibody \/ IQ motif-containing protein M","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIQCM Antibody \/ IQ motif-containing protein M is a anti-IQCM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IQCM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIQCM antibody detects IQ motif-containing protein M, a testis-expressed protein involved in spermatogenesis, cilia formation, and flagellar motility. Encoded by the IQCM gene on chromosome 3q29, this protein contains IQ motifs that mediate calcium-dependent interactions with calmodulin and other regulatory proteins. IQCM plays a critical role in sperm tail formation and motility, contributing to male fertility and normal reproductive function.\u003cbr\u003e\u003cbr\u003eStructurally, IQCM is characterized by its multiple IQ calmodulin-binding motifs, which enable dynamic regulation of protein conformation in response to intracellular calcium levels. It localizes primarily to developing germ cells, particularly in the flagellar basal body and axoneme, suggesting a function in microtubule organization and motor protein regulation. IQCM may interact with dynein and kinesin complexes to ensure coordinated flagellar movement.\u003cbr\u003e\u003cbr\u003eThe IQCM antibody is widely used in reproductive biology, developmental genetics, and ciliary research to examine spermatogenesis, motility regulation, and structural assembly of axonemal complexes. Western blot analysis identifies a 115 kilodalton band corresponding to IQCM, while immunofluorescence shows strong staining in the sperm flagellum and cytoplasmic vesicles of differentiating spermatids. This antibody supports studies exploring the molecular machinery underlying cilia and flagella function.\u003cbr\u003e\u003cbr\u003eMutations or loss of IQCM lead to defective sperm motility and infertility in animal models, emphasizing its essential role in sperm tail formation. The calcium-calmodulin-dependent activity of IQCM links cellular signaling to mechanical movement, integrating metabolic and structural regulation in germ cell development. The IQCM antibody provides an effective reagent for dissecting these interactions at the molecular level.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IQCM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IQCM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IQCM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IQCM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IQCM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303897453,"sku":"FY12852","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ee26dabc-1f8f-4360-ad78-6db756a44458.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/iqcm-antibody-iq-motif-containing-protein-m-bha17135754","provider":"BioHippo","version":"1.0","type":"link"}