{"product_id":"iqsec2-antibody-iq-motif-and-sec7-domain-containing-protein-2-bha17135247","title":"IQSEC2 Antibody \/ IQ motif and Sec7 domain-containing protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIQSEC2 Antibody \/ IQ motif and Sec7 domain-containing protein 2 is a anti-IQSEC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IQSEC2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe IQSEC2 antibody targets IQ motif and Sec7 domain-containing protein 2, a guanine nucleotide exchange factor (GEF) encoded by the IQSEC2 gene. This multidomain protein belongs to the IQSEC\/BRAG family of Arf GEFs, which regulate small GTPases of the ADP-ribosylation factor (Arf) family. IQ motif and Sec7 domain-containing protein 2 is primarily expressed in the brain, where it contributes to synaptic signaling, dendritic spine morphogenesis, and neuronal communication. The IQSEC2 antibody provides a reliable tool to examine the localization, regulation, and signaling roles of this neuron-specific exchange factor.\u003cbr\u003e\u003cbr\u003eIQ motif and Sec7 domain-containing protein 2 contains an IQ motif that binds calmodulin and a Sec7 catalytic domain responsible for activating Arf6. Through these structural features, IQSEC2 regulates membrane trafficking and actin cytoskeleton dynamics at excitatory synapses. Its activity influences AMPA receptor endocytosis, synaptic plasticity, and overall neuronal connectivity. The IQSEC2 antibody enables researchers to visualize these molecular processes, helping clarify how alterations in Arf6 activation impact synaptic function and brain development.\u003cbr\u003e\u003cbr\u003eMutations in IQSEC2 are associated with X-linked intellectual disability, autism spectrum disorder, and epileptic encephalopathy. These pathogenic variants disrupt GEF activity or protein stability, resulting in abnormal neuronal signaling and synapse formation. By using the IQSEC2 antibody, investigators can detect wild-type and mutant protein forms in patient-derived cells, animal models, or neuronal cultures to better understand the molecular pathology underlying neurodevelopmental disorders. Analysis of IQ motif and Sec7 domain-containing protein 2 expression using this antibody provides insight into how altered GTPase signaling contributes to cognitive impairment and epilepsy.\u003cbr\u003e\u003cbr\u003eThe IQSEC2 antibody is suitable for western blotting, immunocytochemistry, and immunohistochemistry applications. It reveals strong neuronal localization, particularly in the postsynaptic density and dendritic compartments. The reagent supports studies on synaptic vesicle recycling, endocytic trafficking, and Arf-dependent signaling. Combined with electrophysiology or live-cell imaging, the IQSEC2 antibody allows correlation of protein expression with functional outcomes in neuronal communication and plasticity.\u003cbr\u003e\u003cbr\u003eBeyond neuroscience, IQ motif and Sec7 domain-containing protein 2 may influence cytoskeletal remodeling in other cell types, including epithelial and immune cells, suggesting broader physiological relevance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IQSEC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IQSEC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IQSEC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IQSEC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IQSEC2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285907821,"sku":"FY12344","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_79bbe4b3-40fe-4ea0-95f5-1c085b5406a7.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/iqsec2-antibody-iq-motif-and-sec7-domain-containing-protein-2-bha17135247","provider":"BioHippo","version":"1.0","type":"link"}