{"product_id":"irak4-antibody-interleukin-1-receptor-associated-kinase-4-bha17135715","title":"IRAK4 Antibody \/ Interleukin-1 receptor-associated kinase 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIRAK4 Antibody \/ Interleukin-1 receptor-associated kinase 4 is a anti-IRAK4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IRAK4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIRAK4 antibody detects Interleukin-1 receptor-associated kinase 4, a serine\/threonine kinase that mediates innate immune signaling through Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). Encoded by the IRAK4 gene on chromosome 12q12, this kinase functions as a central adaptor and activator in MyD88-dependent inflammatory pathways. Upon receptor activation, IRAK4 phosphorylates downstream kinases and recruits TRAF6, leading to activation of NF-kB and MAPK signaling cascades that drive cytokine production and immune defense.\u003cbr\u003e\u003cbr\u003eStructurally, IRAK4 contains an N-terminal death domain that mediates interaction with MyD88 and IRAK1, a kinase domain responsible for autophosphorylation, and regulatory regions that ensure signal specificity. IRAK4 acts as the first kinase activated in the TLR\/IL-1R pathway, enabling the assembly of the Myddosome complex. This signaling cascade is essential for early innate immune responses against bacterial and viral pathogens.\u003cbr\u003e\u003cbr\u003eThe IRAK4 antibody is widely used in immunology, inflammation, and infection research to study signal transduction, cytokine regulation, and kinase activation. Western blot analysis detects a 52 kilodalton band corresponding to IRAK4, while immunofluorescence and immunohistochemistry show cytoplasmic staining in immune and epithelial cells. This antibody supports characterization of TLR signaling and innate immune responses in both normal and pathological states.\u003cbr\u003e\u003cbr\u003eDeficiency or loss-of-function mutations in IRAK4 cause primary immunodeficiency characterized by impaired IL-1 and TLR signaling and recurrent bacterial infections. Conversely, overactivation contributes to chronic inflammation, autoimmune diseases, and cancer. The IRAK4 antibody provides a reliable tool for exploring innate immune signaling and therapeutic kinase targeting.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IRAK4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IRAK4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IRAK4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IRAK4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IRAK4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047302422893,"sku":"FY12813","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9dbb1f64-f47d-4783-a787-9e8c6b9e673e.jpg?v=1782237044","url":"https:\/\/www.ebiohippo.com\/products\/irak4-antibody-interleukin-1-receptor-associated-kinase-4-bha17135715","provider":"BioHippo","version":"1.0","type":"link"}