{"product_id":"irf1-reporter-lentivirus-bhv19400247","title":"IRF1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIRF1 (interferon regulatory factor 1) is a transcription factor that coordinates innate and adaptive immune responses. Activated downstream of pattern-recognition receptors such as Toll-like, RIG-I-like, NOD-like, and C-type lectin receptors, IRF family members translocate to the nucleus and bind interferon-stimulated response element-like sequences. IRF1 induces interferons and immune effector genes, supports antiviral and antibacterial defense, and contributes to immune surveillance against intracellular threats. It also shapes the development and function of NK cells, macrophages, and Th1\/CD8+ T cells. IRF response elements integrate signals from AP-1, IRF, and NF-κB motifs, allowing IRF1 reporters to capture cytokine- and pathogen-driven transcriptional activation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe IRF1 Reporter Lentivirus is a transcription-factor reporter system for detecting IRF1-mediated transcriptional activity. It contains tandem repeats of IRF response elements derived from the human IFNβ enhancer, comprising AP-1, IRF, and NF-κB motifs, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study IRF1 activation in cell-signaling and immunology research. Supplied as purified lentiviral particles.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the IRF1 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"IRF1-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251619619181,"sku":"LTV-0140-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362090246509,"sku":"LTV-0140-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362090279277,"sku":"LTV-0140-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"IRF1-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362090312045,"sku":"LTV-0140-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362090344813,"sku":"LTV-0140-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362090377581,"sku":"LTV-0140-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362090410349,"sku":"LTV-0140-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"IRF1-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362090443117,"sku":"LTV-0140-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"IRF1-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362090475885,"sku":"LTV-0140-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"IRF1-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362090508653,"sku":"LTV-0140-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"IRF1-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362090541421,"sku":"LTV-0140-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IRF1-REPORTER-6P.jpg?v=1776934155","url":"https:\/\/www.ebiohippo.com\/products\/irf1-reporter-lentivirus-bhv19400247","provider":"BioHippo","version":"1.0","type":"link"}