{"product_id":"irf2bp1-antibody-interferon-regulatory-factor-2-binding-protein-1-bha17135666","title":"IRF2BP1 Antibody \/ Interferon regulatory factor 2-binding protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIRF2BP1 Antibody \/ Interferon regulatory factor 2-binding protein 1 is a anti-IRF2BP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IRF2BP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIRF2BP1 antibody detects Interferon regulatory factor 2-binding protein 1, a transcriptional corepressor that modulates interferon signaling and immune gene expression. Encoded by the IRF2BP1 gene on chromosome 19q13.11, this protein functions as a transcriptional regulator by binding and repressing the activity of interferon regulatory factor 2 (IRF2). IRF2BP1 contains a C-terminal zinc finger domain that mediates DNA and protein interactions, as well as an N-terminal coiled-coil region that enables homo- and heterodimerization. It acts as a transcriptional corepressor by recruiting histone deacetylases and other chromatin-modifying enzymes to interferon-stimulated gene promoters.\u003cbr\u003e\u003cbr\u003eIRF2BP1 plays an essential role in maintaining immune homeostasis and regulating the magnitude of antiviral and inflammatory responses. By modulating IRF2 and IRF1 target genes, it fine-tunes the balance between transcriptional activation and repression in interferon signaling pathways. IRF2BP1 also participates in the negative regulation of p53 and NF-?B target genes, thereby linking interferon signaling to cell survival and immune control. Its activity is regulated through post-translational modifications such as phosphorylation and SUMOylation, which alter subcellular localization and cofactor binding.\u003cbr\u003e\u003cbr\u003eThe IRF2BP1 antibody is widely used in immunology, virology, and transcriptional regulation studies to detect IRF2BP1 expression and nuclear localization. Western blot analysis typically identifies a 58 kilodalton band, and immunofluorescence reveals nuclear and perinuclear distribution patterns consistent with its function as a transcriptional repressor. This antibody enables researchers to examine the interplay between interferon signaling and chromatin regulation, as well as transcriptional repression during immune activation.\u003cbr\u003e\u003cbr\u003eDysregulation of IRF2BP1 expression has been associated with aberrant immune responses, chronic inflammation, and oncogenesis. It acts as a checkpoint that restrains excessive interferon responses, preventing autoimmunity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IRF2BP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IRF2BP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IRF2BP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IRF2BP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IRF2BP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298982253,"sku":"FY12763","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0652eacd-697a-42b8-9759-c174811f70ef.jpg?v=1772019398","url":"https:\/\/www.ebiohippo.com\/products\/irf2bp1-antibody-interferon-regulatory-factor-2-binding-protein-1-bha17135666","provider":"BioHippo","version":"1.0","type":"link"}