{"product_id":"isobutyryl-coenzyme-a-free-acid-bhb21200071","title":"Isobutyryl Coenzyme A, Free acid","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eIsobutyryl Coenzyme A, Free acid is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Lyophilized powder, purity ≥ 95%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e Isobutanoyl-CoA, 2-Methylpropionyl-CoA.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C25H42N7O17P3S; 837.62 g\/mol; ≥ 95%. The Free Acid form provides enhanced aqueous stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm \/ Solubility:\u003c\/strong\u003e Lyophilized powder; Soluble in water.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e Isobutanoyl-CoA, 2-Methylpropionyl-CoA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOrigin:\u003c\/strong\u003e Biosynthetic synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eIsobutyryl coenzyme A (Isobutyryl-CoA) is a short-chain branched acyl-CoA intermediate involved in the biosynthesis of various secondary metabolites, including the antibiotic myxalamid B. It serves as the starter unit for the biosynthetic pathway of these compounds and is essential for studies of enzymes like isobutyryl-CoA mutase (EC 5.4.99.13), which catalyzes the interconversion of acyl-CoA derivatives. Isobutyryl-CoA is also pivotal in research exploring acyl-CoA metabolism, as it participates in reactions linked to fatty acid elongation and branching (Berdy, 2005; Stenson, 2008).\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAcyl-CoA metabolism increasingly linked to histone acylation marks and epigenetic regulation in cancer and metabolic disease research.\u003c\/li\u003e\n\u003cli\u003eGrowing interest in short-chain fatty acid CoA thioesters as mediators of gut microbiome–host metabolic crosstalk.\u003c\/li\u003e\n\u003cli\u003eCoA-dependent enzymes investigated as drug targets in infectious disease and neurometabolic disorder research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme kinetics assays — direct substrate for acyltransferases, thiolases, and dehydrogenases.\u003c\/li\u003e\n\u003cli\u003eMetabolic flux analysis — isotope-labeled variants available for stable-isotope tracing.\u003c\/li\u003e\n\u003cli\u003eIn vitro pathway reconstitution for fatty acid β-oxidation, TCA cycle, or polyketide biosynthesis.\u003c\/li\u003e\n\u003cli\u003eBiochemical characterization of CoA-binding proteins by activity assays or binding measurements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCoA thioesters hydrolyze at neutral–alkaline pH; prepare working solutions fresh and keep on ice.\u003c\/li\u003e\n\u003cli\u003eDifferent salt forms share the same core structure — normalize concentrations using the free-acid MW when comparing across forms.\u003c\/li\u003e\n\u003cli\u003eThiol oxidation may occur upon air exposure; use under inert atmosphere or with reducing agents where appropriate.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249726644589,"sku":"A-71-10MG","price":556.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/53dbfa_ef0962bdd86f4687b77200440e23e01b_mv2.png?v=1776855584","url":"https:\/\/www.ebiohippo.com\/products\/isobutyryl-coenzyme-a-free-acid-bhb21200071","provider":"BioHippo","version":"1.0","type":"link"}