{"product_id":"isoc2-antibody-isochorismatase-domain-containing-protein-2-bha17135598","title":"ISOC2 Antibody \/ Isochorismatase domain-containing protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eISOC2 Antibody \/ Isochorismatase domain-containing protein 2 is a anti-ISOC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ISOC2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF, ICC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eISOC2 antibody recognizes Isochorismatase domain-containing protein 2, a cytoplasmic enzyme that belongs to the isochorismatase family of hydrolases. Encoded by the ISOC2 gene on chromosome 19p13.12, this protein contains a conserved catalytic isochorismatase domain that hydrolyzes amide bonds in small-molecule substrates, although its physiological substrates in mammalian cells remain incompletely defined. Structural and proteomic studies indicate that ISOC2 may participate in amino acid metabolism, mitochondrial function, and redox homeostasis. The protein localizes to the cytosol and mitochondrial matrix, where it interacts with metabolic enzymes and stress-response factors. Its expression is ubiquitous but highest in metabolically active tissues such as liver, heart, and skeletal muscle.\u003cbr\u003e\u003cbr\u003eISOC2 is evolutionarily conserved from bacteria to humans, suggesting an ancestral enzymatic role in intermediate metabolism. While the bacterial isochorismatases catalyze the hydrolysis of isochorismate to 2,3-dihydroxybenzoate in siderophore biosynthesis, mammalian ISOC2 lacks this function but retains the characteristic aspartate and cysteine residues that form the catalytic dyad. Structural modeling implies that ISOC2 might act on endogenous metabolites involved in oxidative stress pathways or NAD-dependent reactions. Transcriptomic profiling has linked ISOC2 expression to mitochondrial biogenesis and adaptive responses to nutrient deprivation.\u003cbr\u003e\u003cbr\u003eThe ISOC2 antibody is used to study mitochondrial metabolism, oxidative stress, and protein-protein interactions involving metabolic enzymes. Western blot analysis detects a 30 kilodalton band corresponding to the mature ISOC2 protein, while immunofluorescence shows diffuse cytoplasmic and mitochondrial staining. Elevated ISOC2 expression has been reported in various cancers, including hepatocellular and colorectal carcinoma, where it may contribute to metabolic reprogramming and proliferation. Silencing of ISOC2 in cancer cell lines reduces ATP production and induces apoptosis, indicating its potential role in maintaining energy homeostasis under stress conditions.\u003cbr\u003e\u003cbr\u003eIn developmental contexts, ISOC2 expression increases during myogenesis and cardiogenesis, reflecting its involvement in energy-demanding differentiation processes. The protein may interact with metabolic regulators such as PGC-1alpha and AMPK to coordinate mitochondrial turnover and antioxidant defense. Because of these broad metabolic implications, the ISOC2 antibody is valuable for basic and translational research in metabolism, oncology, and mitochondrial biology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ISOC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ISOC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ISOC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ISOC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ISOC2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296557421,"sku":"FY12695","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_28ee1859-893f-4d2d-887c-ed8014140131.jpg?v=1782237032","url":"https:\/\/www.ebiohippo.com\/products\/isoc2-antibody-isochorismatase-domain-containing-protein-2-bha17135598","provider":"BioHippo","version":"1.0","type":"link"}